Fine Mapping and Candidate Gene Analysis of BnC08.cds, a Recessive Gene Responsible for Sepal-Specific Chlorophyll-Deficiency in Brassica napus L.
Fine Mapping and Candidate Gene Analysis of BnC08.cds, a Recessive Gene Responsible for Sepal-Specific Chlorophyll-Deficiency in Brassica napus L.
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甘蓝型油菜萼片特异性叶绿素缺乏隐性基因BnC08.cds的精细定位及候选基因分析
DOI：
10.3389/fpls.2022.850330
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发表时间：
2022
影响因子：
5.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Zhang W;Zhou X;Chen F;Zhu H;Shi R;Sun C;Chen S;Hu M;Zhang J;Wang X
叶绿体发育对光合作用和植物生长至关重要，其调控涉及多种因素。不同绿色组织的调控机制不同，以往的研究主要集中在叶片的叶绿体上。本研究在甘蓝型油菜中发现了一个萼片特异性叶绿素缺乏突变体，命名为df74。遗传分析表明，该表型受单隐性核基因控制。通过全基因组测序，将该基因定位在C08染色体上，命名为BnC08.cds。绘制BnC08的精细图。以df74与中双11 （ZS11）杂交，获得一个F2群体。最后是BnC08。cds在单核苷酸多态性（SNP）标记M5和M6之间的区域精细定位，对应于甘蓝型油菜ZS11基因组228.72 kb的区间。在靶区预测了18个基因，根据转录组分析和序列变异分析结果推测BnaC08G0442100ZS是最有可能的候选基因。这些结果为探讨花萼叶绿体发育的调控机制提供了基础。
Chloroplast development is crucial for photosynthesis and plant growth and many factors are involved in its regulation. The regulatory mechanism differs in different green tissues, and previous studies have focused on chloroplasts in leaves. In this study, a mutant with sepal-specific chlorophyll-deficiency was observed in Brassica napus and named as df74. Genetic analysis indicated that the phenotype was controlled by a single recessive nuclear gene. The gene was located on chromosome C08 by bulked-segregant analysis with whole-genome sequencing, which was designated as BnC08.cds. To fine-map the BnC08.cds, a F2 population was created from the cross of df74 and Zhongshuang11 (ZS11). Finally, the BnC08.cds was fine-mapped in the region between the single-nucleotide polymorphism (SNP) markers M5 and M6, corresponding to a 228.72 kb interval of the B. napus “ZS11” genome. Eighteen genes were predicted in the target region, and it was speculated that BnaC08G0442100ZS was the most likely candidate gene based on the results of transcriptome analyses and sequence variation analyses. These results provide a foundation to explore the regulation of chloroplast development in sepals.
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影响因子：
4.8
作者：
Qin Yaobin;Cheng Peng;Ying Jiezheng
通讯作者：
Ying Jiezheng
DOI：
10.1111/j.1365-313x.2011.04814.x
发表时间：
2012-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Pattanayak GK;Venkataramani S;Hortensteiner S;Kunz L;Christ B;Moulin M;Smith AG;Okamoto Y;Tamiaki H;Sugishima M;Greenberg JT
通讯作者：
Greenberg JT
影响因子：
5.1
作者：
LIM, SH;WITTY, M;SMITH, AG
通讯作者：
SMITH, AG
影响因子：
6.9
作者：
Ma F;Hu Y;Ju Y;Jiang Q;Cheng Z;Zhang Q;Sodmergen
通讯作者：
Sodmergen
lcm1基因的精细定位，该基因赋予大白菜（Brassica rapa ssp. pekinensis）缺乏叶绿素的金黄色叶子
影响因子：
3.1
作者：
Fu, Wei;Ye, Xueling;Liu, Zhiyong
通讯作者：
Liu, Zhiyong