Characterizing Deformability of Drug Resistant Patient-Derived Acute Lymphoblastic Leukemia (ALL) Cells Using Acoustic Tweezers.
Characterizing Deformability of Drug Resistant Patient-Derived Acute Lymphoblastic Leukemia (ALL) Cells Using Acoustic Tweezers.
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DOI：
10.1038/s41598-018-34024-3
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发表时间：
2018-10-24
影响因子：
4.6
通讯作者：
Kim YM
中科院分区：
文献类型：
--
作者：
Liu HC;Gang EJ;Kim HN;Lim HG;Jung H;Chen R;Abdel-Azim H;Shung KK;Kim YM
细胞力学在癌细胞中的作用是一个新的研究领域，已经导致了新的治疗抗性机制的鉴定。单束声镊是一种很有前途的细胞力学表型定量技术。我们以前的研究表明，SBA镊子可以用来量化贴壁乳腺癌细胞系的变形能力。涉及化疗耐药性的患者来源的（原代）前B急性淋巴细胞白血病（ALL）细胞的物理特性尚未得到广泛研究。在这里，我们证明了使用SBA镊子从四个病例中分析原代前B ALL细胞的可行性。ALL细胞表现出随着SBA镊子声压的增加而增加的变形能力。此外，对化疗药物有抗性的ALL细胞比未治疗的ALL细胞更易变形。我们证明了SBA镊子可以量化非粘附性白血病细胞的变形能力，并以无接触和无标记的方式在化疗耐药白血病细胞中区分这种机械表型。
The role of cell mechanics in cancer cells is a novel research area that has resulted in the identification of new mechanisms of therapy resistance. Single beam acoustic (SBA) tweezers are a promising technology for the quantification of the mechanical phenotype of cells. Our previous study showed that SBA tweezers can be used to quantify the deformability of adherent breast cancer cell lines. The physical properties of patient-derived (primary) pre-B acute lymphoblastic leukemia (ALL) cells involved in chemotherapeutic resistance have not been widely investigated. Here, we demonstrate the feasibility of analyzing primary pre-B ALL cells from four cases using SBA tweezers. ALL cells showed increased deformability with increasing acoustic pressure of the SBA tweezers. Moreover, ALL cells that are resistant to chemotherapeutic drugs were more deformable than were untreated ALL cells. We demonstrated that SBA tweezers can quantify the deformability of nonadherent leukemia cells and discriminate this mechanical phenotype in chemotherapy-resistant leukemia cells in a contact- and label-free manner.
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