Probing mechanical properties of Jurkat cells under the effect of ART using oscillating optical tweezers.
Probing mechanical properties of Jurkat cells under the effect of ART using oscillating optical tweezers.
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DOI：
10.1371/journal.pone.0126548
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Park EJ
中科院分区：
文献类型：
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作者：
Khakshour S;Beischlag TV;Sparrey C;Park EJ
急性淋巴细胞白血病是一种常见的血癌，化疗是首选的初始治疗方法。在细胞水平上量化化疗药物的作用在治疗过程中起着重要的作用。在这项研究中，使用振荡光镊来表征暴露于化疗药物青蒿琥酯（ART）的Jurkat细胞的频率依赖性机械特性。应用一个与细胞结合的头的运动方程来描述细胞骨架的力学特性。通过光学镊子的模拟结果与实验结果的比较，得到了ART处理前后Jurkat细胞的刚度和粘度。结果表明，单元刚度与频率呈弱幂律关系。此外，处理后的硬度和粘度都有所增加。因此，细胞骨架细胞刚度以及幂律系数可以为药物治疗癌细胞的化学-力学关系提供有用的见解，并可作为定量评估治疗效果的另一种工具。
Acute lymphoid leukemia is a common type of blood cancer and chemotherapy is the initial treatment of choice. Quantifying the effect of a chemotherapeutic drug at the cellular level plays an important role in the process of the treatment. In this study, an oscillating optical tweezer was employed to characterize the frequency-dependent mechanical properties of Jurkat cells exposed to the chemotherapeutic agent, artesunate (ART). A motion equation for a bead bound to a cell was applied to describe the mechanical characteristics of the cell cytoskeleton. By comparing between the modeling results and experimental results from the optical tweezer, the stiffness and viscosity of the Jurkat cells before and after the ART treatment were obtained. The results demonstrate a weak power-law dependency of cell stiffness with frequency. Furthermore, the stiffness and viscosity were increased after the treatment. Therefore, the cytoskeleton cell stiffness as the well as power-law coefficient can provide a useful insight into the chemo-mechanical relationship of drug treated cancer cells and may serve as another tool for evaluating therapeutic performance quantitatively.
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