Genomic characterization of Haemophilus parasuis SH0165, a highly virulent strain of serovar 5 prevalent in China.
Genomic characterization of Haemophilus parasuis SH0165, a highly virulent strain of serovar 5 prevalent in China.
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中国流行的 5 型高毒力副猪嗜血杆菌 SH0165 的基因组特征
DOI：
10.1371/journal.pone.0019631
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Chen H
中科院分区：
文献类型：
--
作者：
Xu Z;Yue M;Zhou R;Jin Q;Fan Y;Bei W;Chen H
副猪嗜血杆菌可以是猪呼吸道的共生细菌，也可以是引起格拉瑟氏病的机会病原体，格拉瑟氏病是一种严重的全身性疾病，已导致全世界养猪业遭受重大经济损失。我们确定了副猪嗜血菌 SH0165 的完整基因组序列，这是一种高毒力血清型 5 菌株，从中国北方的一个猪圈中分离出来。单个环状染色体的长度为 2,269,156 个碱基对，包含 2,031 个蛋白质编码基因。结合代谢途径分析检测到的全谱基因，我们证实副猪嗜血菌通过发酵和呼吸产生 ATP，并具有完整的 TCA 合成代谢循环。除了拥有血红素生物合成所必需的完整途径外，该病原体还被发现配备有不同的铁获取系统，例如 TonB 系统和 ABC 型转运复合物，以克服感染和持续期间的铁限制。我们鉴定了许多编码潜在毒力因子的基因，例如 IV 型菌毛和表面多糖。基因组分析证实副猪嗜血菌具有天然能力，因为存在与 DNA 摄取相关的基因。在 SH0165 基因组中发现了一个九聚体 DNA 摄取信号序列 (ACAAGCGGT)，与胸膜肺炎放线杆菌和溶血曼海姆氏菌中发现的序列相同，后面有类似的下游基序。与其他巴氏杆菌科物种的基因组和系统发育比较进一步表明，副猪嗜血菌与另一种猪病原菌胸膜肺炎放线菌密切相关。这里提出的综合遗传分析为未来研究副猪嗜血菌的代谢、自然能力和毒力奠定了基础。
Haemophilus parasuis can be either a commensal bacterium of the porcine respiratory tract or an opportunistic pathogen causing Glässer's disease, a severe systemic disease that has led to significant economical losses in the pig industry worldwide. We determined the complete genomic sequence of H. parasuis SH0165, a highly virulent strain of serovar 5, which was isolated from a hog pen in North China. The single circular chromosome was 2,269,156 base pairs in length and contained 2,031 protein-coding genes. Together with the full spectrum of genes detected by the analysis of metabolic pathways, we confirmed that H. parasuis generates ATP via both fermentation and respiration, and possesses an intact TCA cycle for anabolism. In addition to possessing the complete pathway essential for the biosynthesis of heme, this pathogen was also found to be well-equipped with different iron acquisition systems, such as the TonB system and ABC-type transport complexes, to overcome iron limitation during infection and persistence. We identified a number of genes encoding potential virulence factors, such as type IV fimbriae and surface polysaccharides. Analysis of the genome confirmed that H. parasuis is naturally competent, as genes related to DNA uptake are present. A nine-mer DNA uptake signal sequence (ACAAGCGGT), identical to that found in Actinobacillus pleuropneumoniae and Mannheimia haemolytica, followed by similar downstream motifs, was identified in the SH0165 genome. Genomic and phylogenetic comparisons with other Pasteurellaceae species further indicated that H. parasuis was closely related to another swine pathogenic bacteria A. pleuropneumoniae. The comprehensive genetic analysis presented here provides a foundation for future research on the metabolism, natural competence and virulence of H. parasuis.
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影响因子：
3.3
作者：
Bigas, A;Garrido, ME;Llagostera, M
通讯作者：
Llagostera, M
影响因子：
3.6
作者：
Brondijk, THC;Fiegen, D;Cole, JA
通讯作者：
Cole, JA
影响因子：
3.2
作者：
Challacombe, Jean F.;Duncan, A. J.;Inzana, Thomas J.
通讯作者：
Inzana, Thomas J.
影响因子：
--
作者：
del Río, ML;Gutiérrez-Martín, CB;Rodríguez-Ferri, EF
通讯作者：
Rodríguez-Ferri, EF
影响因子：
3.9
作者：
Dailey, HA
通讯作者：
Dailey, HA