Spatiotemporal expression of regulatory kinases directs the transition from mitosis to cellular morphogenesis in Drosophila.
Spatiotemporal expression of regulatory kinases directs the transition from mitosis to cellular morphogenesis in Drosophila.
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DOI：
10.1038/s41467-022-28322-8
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发表时间：
2022-02-09
影响因子：
16.6
通讯作者：
Johnson AN
中科院分区：
文献类型：
--
作者：
Yang S;McAdow J;Du Y;Trigg J;Taghert PH;Johnson AN
胚胎发生依赖于有丝分裂、分化和形态发生之间严格调节的平衡。了解胚胎如何使用相对少量的蛋白质在生长和形态发生之间过渡是发育生物学的中心问题，但控制有丝分裂和分化的机制被认为是根本不同的。在这里，我们表明有丝分裂激酶波罗，调节果蝇有丝分裂的所有步骤，也指导细胞周期退出后的细胞形态发生。在有丝分裂细胞中，极光激酶激活波罗来控制指导胞质分裂的细胞骨架调节模块。我们发现，在有丝分裂后的中胚层，控制波罗活动的极光激酶过渡到未表征的激酶后座司机（BSD），其中BSD和波罗合作，以调节肌肉形态发生。因此，波罗及其效应物指导有丝分裂和细胞形态发生，但从生长到形态发生的转变由上游活化激酶的时空表达决定。在发育过程中调节有丝分裂和分化的机制被认为是不同的。在这里，他们表明，在果蝇有丝分裂激酶波罗调节细胞周期退出后的细胞形态发生。
Embryogenesis depends on a tightly regulated balance between mitosis, differentiation, and morphogenesis. Understanding how the embryo uses a relatively small number of proteins to transition between growth and morphogenesis is a central question of developmental biology, but the mechanisms controlling mitosis and differentiation are considered to be fundamentally distinct. Here we show the mitotic kinase Polo, which regulates all steps of mitosis in Drosophila, also directs cellular morphogenesis after cell cycle exit. In mitotic cells, the Aurora kinases activate Polo to control a cytoskeletal regulatory module that directs cytokinesis. We show that in the post-mitotic mesoderm, the control of Polo activity transitions from the Aurora kinases to the uncharacterized kinase Back Seat Driver (Bsd), where Bsd and Polo cooperate to regulate muscle morphogenesis. Polo and its effectors therefore direct mitosis and cellular morphogenesis, but the transition from growth to morphogenesis is determined by the spatiotemporal expression of upstream activating kinases. The mechanisms regulating mitosis and differentiation during development are thought to be distinct. Here they show that in Drosophila the mitotic kinase Polo regulates cellular morphogenesis after cell cycle exit.
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影响因子：
11.8
作者：
Bustos F;Segarra-Fas A;Nardocci G;Cassidy A;Antico O;Davidson L;Brandenburg L;Macartney TJ;Toth R;Hastie CJ;Moran J;Gourlay R;Varghese J;Soares RF;Montecino M;Findlay GM
通讯作者：
Findlay GM
影响因子：
64.5
作者：
BLAU, HM;CHIU, CP;WEBSTER, C
通讯作者：
WEBSTER, C
影响因子：
11.8
作者：
Cabral, Gabriela;Laos, Triin;Dammermann, Alexander
通讯作者：
Dammermann, Alexander
影响因子：
4.8
作者：
Ebrahimi, Saman;Fraval, Hamilton;Gregory, Stephen L.
通讯作者：
Gregory, Stephen L.
影响因子：
7.8
作者：
Carmena, M;Riparbelli, MG;Minestrini, G;Tavares, AM;Adams, R;Callaini, G;Glover, DM
通讯作者：
Glover, DM