Identification of Actinobacillus actinomycetemcomitans by leukotoxin gene-specific hybridization and polymerase chain reaction assays
Identification of Actinobacillus actinomycetemcomitans by leukotoxin gene-specific hybridization and polymerase chain reaction assays
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通过白细胞毒素基因特异性杂交和聚合酶链反应测定鉴定伴放线放线杆菌
DOI：
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发表时间：
1993
影响因子：
9.4
通讯作者：
R. Haas
中科院分区：
文献类型：
--
作者：
T. Tønjum;R. Haas
以白细胞毒素基因为靶标，通过基因检测鉴定出从全身感染、局部脓肿和牙周炎病例中分离出的 11 株放线放线杆菌。我们开发了一种基于该病原体的白细胞毒素结构基因的聚合酶链反应 (PCR) 检测方法，该检测方法清楚地鉴定了所有测试的 A. actinomycetemcomitans 菌株，并将它们与密切相关的嗜血嗜血杆菌以及其他细菌物种分开。此外，用克隆的部分白细胞毒素结构基因（lktA）作为探针进行DNA-DNA杂交，这再次清楚地区分了A. actinomycetemcomitans与H. aphrophilus、部分正常口腔菌群以及含有RTX（毒素重复）家族相关细胞毒素的物种。当在杂交实验中用作探针时，从白细胞毒素结构基因扩增的PCR片段给出的结果与克隆的白细胞毒素基因给出的结果相似。这里描述的杂交和 PCR 测定是鉴定 A. actinomycetemcomitans 的根本性改进。
Eleven strains of Actinobacillus actinomycetemcomitans isolated from cases of systemic infections, local abscesses, and periodontitis were identified by genetic assays using the leukotoxin gene as the target. We have developed a polymerase chain reaction (PCR) assay, based on the leukotoxin structural gene of this pathogen, which clearly identified all tested strains of A. actinomycetemcomitans and separated them from the closely related Haemophilus aphrophilus as well as other bacterial species. Furthermore, DNA-DNA hybridization was performed with the cloned partial leukotoxin structural gene (lktA) as a probe, which again clearly distinguished A. actinomycetemcomitans from H. aphrophilus, parts of the normal oral flora, and species harboring RTX (repeats in toxin) family-related cytotoxins. The PCR fragment amplified from the leukotoxin structural gene gave results similar to those given by the cloned leukotoxin gene when used as a probe in hybridization experiments. The hybridization and PCR assays described here are fundamental improvements for the identification of A. actinomycetemcomitans.
伴放线放线杆菌白毒素基因的分析。
DOI：
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发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Lally,ET;Golub,EE;Kieba,IR;Taichman,NS;Rosenbloom,J;Rosenbloom,JC;Gibson,CW;Demuth,DR
通讯作者：
Demuth,DR
通过多位点酶电泳区分放线杆菌（嗜血杆菌）放线菌、嗜血嗜血杆菌和副嗜血杆菌。
DOI：
10.1099/00221287-136-10-2135
发表时间：
1990
期刊：
Journal of general microbiology
影响因子：
--
作者：
Caugant,DA;Selander,RK;Olsen,I
通讯作者：
Olsen,I
通过聚类分析和脱氧核糖核酸杂交对伴放线放线杆菌和嗜血嗜血杆菌进行分类和鉴定。
影响因子：
3.5
作者：
Tanner,AC;Visconti,RA;Socransky,SS;Holt,SC
通讯作者：
Holt,SC