Nonlinear optical corneal collagen crosslinking of ex vivo rabbit eyes.
Nonlinear optical corneal collagen crosslinking of ex vivo rabbit eyes.
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DOI：
10.1016/j.jcrs.2016.06.040
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发表时间：
2016-11
影响因子：
2.8
通讯作者：
Jester JV
中科院分区：
文献类型：
--
作者：
Bradford SM;Brown DJ;Juhasz T;Mikula E;Jester JV
确定是否可以在离体兔眼角膜基质中使用低数值孔径透镜和扩大焦点体积的非线性光学激发精确地实现核黄素诱导的胶原交联（CXL）。加文赫伯特眼科研究所，加州尔湾大学，尔湾，加州，美国。实验研究。去除角膜上皮，将角膜浸泡在0.5%核黄素溶液中。使用0.1数值孔径物镜，使用1 W的760 nm飞秒激光和光栅扫描产生150 μm × 3 μm的理论激发体积，其中光栅以4.4 μm的线间距以不同的有效速度在4.50 mm × 2.25 mm的区域上扫描。检查角膜切片的胶原自发荧光。胶原蛋白的自发荧光比UVA CXL增强2.9倍。此外，增加的速度与降低的自发荧光强度呈线性相关。最慢速度为2.69 mm/s，在中央角膜轴向显示平均182.97 μm ± 52.35（SD）长的自体荧光扫描线，而UVA CXL为147.84 ± 4.35 μm。减少停留时间与减少自发荧光强度线性相关，以8.9 mm/s的速度接近UVA CXL的自发荧光强度。使用8.9 mm/s的有效速度，可以在不到4分钟的时间内在3.0 mm直径的区域上实现非线性光学CXL。非线性光学CXL的进一步发展可能会导致更安全，更快，更有效的CXL治疗。
To determine whether riboflavin-induced collagen crosslinking (CXL) could be precisely achieved in the corneal stroma of ex vivo rabbit eyes using nonlinear optical excitation with a low numerical aperture lens and enlarged focal volume. Gavin Herbert Eye Institute, University of California Irvine, Irvine, California, USA. Experimental study. The corneal epithelium was removed and the corneas were soaked in 0.5% riboflavin solution. Using a 0.1 numerical aperture objective, a theoretical excitation volume of 150 μm × 3 μm was generated using 1 W of 760 nm femtosecond laser light and raster scanned with 4.4 μm line separation at varying effective speeds over a 4.50 mm × 2.25 mm area. Corneal sections were examined for collagen autofluorescence. Collagen autofluorescence was enhanced 2.9 times compared with ultraviolet-A (UVA) CXL. Also, increasing speed was linearly associated with decreasing autofluorescence intensity. The slowest speed of 2.69 mm/s showed a mean of 182.97 μm ± 52.35 (SD) long autofluorescent scan lines axially in the central cornea compared with 147.84 ± 4.35 μm for UVA CXL. Decreasing dwell time was linearly associated with decreasing autofluorescence intensity, approaching that of UVA CXL at a speed of 8.9 mm/s. Using an effective speed of 8.9 mm/s, nonlinear optical CXL could be achieved over a 3.0 mm diameter area in fewer than 4 minutes. Further development of nonlinear optical CXL might result in safer, faster, and more effective CXL treatments.
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