Immunoproteomic to analysis the pathogenicity factors in leukopenia caused by Klebsiella pneumonia bacteremia.
Immunoproteomic to analysis the pathogenicity factors in leukopenia caused by Klebsiella pneumonia bacteremia.
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免疫蛋白质组学分析肺炎克雷伯菌菌血症所致白细胞减少症的致病因素
DOI：
10.1371/journal.pone.0110011
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Zhou Z
中科院分区：
文献类型：
--
作者：
Liu H;Cheng Z;Song W;Wu W;Zhou Z
由菌血症引起的白细胞减少发生率显著增加，并与住院时间延长和费用增加有关。免疫蛋白质组学是一种很有前途的方法来鉴定不同疾病的致病因素。本研究采用免疫蛋白组学方法分析肺炎克雷伯菌菌血症所致白细胞减少的致病因素。在双向电泳凝胶上检测到大约40个定位于4 ~ 7 pI范围的蛋白点，鉴定出6个在10 ~ 170 kDa之间的差异表达蛋白点。致病因子包括s -腺苷甲硫氨酸合成酶、丙酮酸脱氢酶、谷胱甘肽合成酶、udp -半乳糖-4- epimase、醋酸激酶A和延伸因子tu （EF-Tu）。为了验证致病性因子，我们使用western blotting显示肺炎克雷伯菌在伴有白细胞减少而不是白细胞增多时具有较高的（EF-Tu）表达。因此，我们报告了肺炎克雷伯菌菌血症引起白细胞减少的6个致病因子，包括5个管家酶和EF-Tu。我们认为EF-Tu可能是肺炎克雷伯菌所致白细胞减少的潜在致病因子。
Incidences of leukopenia caused by bacteremia have increased significantly and it is associated with prolonged hospital stay and increased cost. Immunoproteomic is a promising method to identify pathogenicity factors of different diseases. In the present study, we used immunoproteomic to analysis the pathogenicity factors in leukopenia caused by Klebsiella Pneumonia bacteremia. Approximately 40 protein spots localized in the 4 to 7 pI range were detected on two-dimensional electrophoresis gels, and 6 differentially expressed protein spots between 10 and 170 kDa were identified. Pathogenicity factors including S-adenosylmethionine synthetase, pyruvate dehydrogenase, glutathione synthetase, UDP-galactose-4-epimerase, acetate kinase A and elongation factor tu (EF-Tu). In validation of the pathogenicity factor, we used western blotting to show that Klebsiella pneumonia had higher (EF-Tu) expression when they accompanied by leukopenia rather than leukocytosis. Thus, we report 6 pathogenicity factors of leukopenia caused by Klebsiella pneumonia bacteremia, including 5 housekeeping enzymes and EF-Tu. We suggest EF-Tu could be a potential pathogenicity factor for leukopenia caused by Klebsiella pneumonia.
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影响因子：
4.4
作者：
DelVecchio, Vito G.;Connolly, Joseph P.;Mujer, Cesar V.
通讯作者：
Mujer, Cesar V.
影响因子：
3.2
作者：
Ferguson, GP;Booth, IR
通讯作者：
Booth, IR
影响因子：
4.5
作者：
Cosgrove, SE;Qi, YL;Carmeli, Y
通讯作者：
Carmeli, Y
影响因子：
3.2
作者：
Lawrence, SH;Ferry, JG
通讯作者：
Ferry, JG
DOI：
10.1007/s10096-004-1156-y
发表时间：
2004-07-01
影响因子：
4.5
作者：
von Lilienfeld-Toal, M;Dietrich, MP;Stuber, F
通讯作者：
Stuber, F