Comparison of lipooligosaccharides from human challenge strains of Neisseria gonorrhoeae.
Comparison of lipooligosaccharides from human challenge strains of Neisseria gonorrhoeae.
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DOI：
10.3389/fmicb.2023.1215946
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发表时间：
2023
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
The alarming rise of antibiotic resistance and the emergence of new vaccine technologies have increased the focus on vaccination to control gonorrhea. Neisseria gonorrhoeae strains FA1090 and MS11 have been used in challenge studies in human males. We used negative-ion MALDI-TOF MS to profile intact lipooligosaccharide (LOS) from strains MS11mkA, MS11mkC, FA1090 A23a, and FA1090 1-81-S2. The MS11mkC and 1-81-S2 variants were isolated from male volunteers infected with MS11mkA and A23a, respectively. LOS profiles were obtained after purification using the classical phenol water extraction method and by microwave-enhanced enzymatic digestion, which is more amenable for small-scale work. Despite detecting some differences in the LOS profiles, the same major species were observed, indicating that microwave-enhanced enzymatic digestion is appropriate for MS studies. The compositions determined for MS11mkA and mkC LOS were consistent with previous reports. FA1090 is strongly recognized by mAb 2C7, an antibody-binding LOS with both α- and β-chains if the latter is a lactosyl group. The spectra of the A23a and 1-81-S2 FA1090 LOS were similar to each other and consistent with the expression of α-chain lacto-N-neotetraose and β-chain lactosyl moieties that can both be acceptor sites for sialic acid substitution. 1-81-S2 LOS was analyzed after culture with and without media supplemented with cytidine-5'-monophosphate N-acetylneuraminic acid (CMP-Neu5Ac), which N. gonorrhoeae needs to sialylate its LOS. LOS sialylation reduces the infectivity of gonococci in men, although it induces serum resistance in serum-sensitive strains and reduces killing by neutrophils and antimicrobial peptides. The infectivity of FA1090 in men is much lower than that of MS11mkC, but the reason for this difference is unclear. Interestingly, some peaks in the spectra of 1-81-S2 LOS after bacterial culture with CMP-Neu5Ac were consistent with disialylation of the LOS, which could be relevant to the reduced infectivity of FA1090 in men and could have implications regarding the phase variation of the LOS and the natural history of infection.
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DOI：
10.4049/jimmunol.1000953
发表时间：
2010-12-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Liu M;John CM;Jarvis GA
通讯作者：
Jarvis GA
DOI：
10.1007/978-1-4939-9496-0_25
发表时间：
2019-01-01
期刊：
NEISSERIA GONORRHOEAE: METHODS AND PROTOCOLS
影响因子：
--
作者：
Hobbs, Marcia M.;Duncan, Joseph A.
通讯作者：
Duncan, Joseph A.
DOI：
10.1073/pnas.95.18.10872
发表时间：
1998-09-01
影响因子：
11.1
作者：
Banerjee, A;Wang, R;Stein, DC
通讯作者：
Stein, DC
影响因子：
6.4
作者：
Gulati, Sunita;Pennington, Michael W.;Rice, Peter A.
通讯作者：
Rice, Peter A.
影响因子：
5.2
作者：
Kahler CM;Nawrocki KL;Anandan A;Vrielink A;Shafer WM
通讯作者：
Shafer WM