Effect of acupuncture on the TLR2/4-NF-κB signalling pathway in a rat model of traumatic brain injury.
Effect of acupuncture on the TLR2/4-NF-κB signalling pathway in a rat model of traumatic brain injury.
复制标题
针刺对脑外伤模型大鼠TLR2/4-NF-κB信号通路的影响
DOI：
10.1136/acupmed-2017-011472
复制
发表时间：
2018-08
期刊：
影响因子：
--
通讯作者：
Zhang YM
中科院分区：
文献类型：
--
作者：
Lin SJ;Cao LX;Cheng SB;Dai QF;Lin JH;Pu L;Chen WH;Zhang YJ;Chen SL;Zhang YM
目的：研究针刺对创伤性脑损伤（traumatic brain injury，TBI）大鼠皮层TLR 2/4-NF-κB信号通路的影响，探讨针刺促进瘢痕修复的可能机制。采用Feeney的自由落体硬膜外撞击模型建立TBI。将108只大鼠随机分为正常对照组（n=18）、TBI模型组（n=36）和TBI+MA组（n=36）。每组又分为3个时间组：3d、7 d和14 d。正常组和TBI组大鼠不作任何处理。TBI+MA组在GV 20、GV 26、GV 16至GV 15和双侧LI 4处进行手针治疗。采用实时荧光定量聚合酶链反应（qPCR）和蛋白质印迹法（Western blot）检测大鼠皮质中TLR 2、TLR 4、NF-κB和蛋白质的mRNA表达。TBI+MA组造模后12 h改良神经功能缺损评分（mNSS）较基线评分改善，7 d和14 d较TBI组改善（P<0.05），而TBI组直到14 d才较基线改善。TBI组TLR 2、TLR 4和NF-κB的mRNA和蛋白表达在3d均高于正常组（P<0.05），7 d达高峰，14 d开始下降。TBI+MA组TLR 2、TLR 4和NF-κB的mRNA和蛋白表达在3d时高于TBI组（P<0.05），7 d时明显下调（P<0.01），14 d时降至正常水平。针刺对TBI大鼠皮层TLR 2/4-NF-κB信号通路相关基因TLR 2、TLR 4和NF-κB具有双向调节作用，早期促进其表达，晚期抑制其表达。
To study the effect of acupuncture on the TLR2/4-NF-κB signalling pathway in the cortex of Sprague-Dawley rats following traumatic brain injury (TBI), and investigate the possible mechanism underlying the effects of acupuncture on scar repair. TBI was established using Feeney’s free-falling epidural percussion model. In total, 108 rats were randomly divided into a normal group (n=18), untreated TBI model group (TBI group, n=36) and manual acupuncture-treated TBI group (TBI+MA, n=36). Each group of rats was subdivided into three time groups: 3-day (3d), 7-day (7d) and 14-day (14d). No treatment was given to rats in the normal and TBI groups. The TBI+MA group received manual acupuncture at GV20, GV26, GV16 through GV15, and bilateral LI4. mRNA expression of TLR2, TLR4, NF-κB and protein in the rat cortices was quantified using real-time fluorescence quantitative polymerase chain reaction (qPCR) and Western blot analyses. The modified neurological severity score (mNSS) scores of the TBI+MA group were improved compared with baseline scores 12 hours after modelling, and improved at 7d and 14d compared with the TBI group (P<0.05), while the score of the TBI group did not improve until 14d compared to baseline. mRNA and protein expression of TLR2, TLR4 and NF-κB in the TBI group were higher than the normal group at 3d (P<0.05), reached a peak at 7d, then began to decrease at 14d. mRNA and protein expression of TLR2, TLR4 and NF-κB were higher in the TBI+MA group compared with the TBI group at 3d (P<0.05), were significantly down-regulated at 7d (P<0.01), and decreased to normal levels at 14d. Acupuncture has a bidirectional regulatory effect on the TLR2/4-NF-κB signalling pathway-related genes TLR2, TLR4 and NF-κB in the TBI rat cortex, promoting their expression in the early stage and inhibiting it in the later stage.
登录
查看更多内容
影响因子：
2.6
作者：
Liu Y;Liu Z;Li X;Luo B;Xiong J;Gan W;Jiang M;Zhang Z;Schluesener HJ;Zhang Z
通讯作者：
Zhang Z
影响因子：
3
作者：
Lu, M;Chen, JL;Chopp, M
通讯作者：
Chopp, M
影响因子：
3.8
作者：
Wang, Jun;Zhao, Hui;Wu, Gen-Cheng
通讯作者：
Wu, Gen-Cheng
影响因子：
5.3
作者：
Burda JE;Bernstein AM;Sofroniew MV
通讯作者：
Sofroniew MV
影响因子：
2.4
作者：
Cuthbert, Jeffrey P.;Harrison-Felix, Cynthia;Whiteneck, Gale G.
通讯作者：
Whiteneck, Gale G.