Release of pyrophosphate by normal mammalian articular hyaline and fibrocartilage in organ culture.

Release of pyrophosphate by normal mammalian articular hyaline and fibrocartilage in organ culture.
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器官培养中正常哺乳动物关节透明和纤维软骨释放焦磷酸盐。

DOI:
10.1002/art.1780241211
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发表时间:
1981
影响因子:
--
通讯作者:
McCarty,DJ
McCarty,DJ
中科院分区:
--
文献类型:
--
作者:
Ryan,LM;Cheung,HS;McCarty,DJ

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二水焦磷酸钙晶体最常见于纤维软骨组织中,较少见于透明关节软骨中。以往的研究发现,焦磷酸盐(PPi)释放到培养基中的未成熟的兔透明软骨和骨关节炎的人软骨在器官培养,而不是由正常的人或成熟的兔软骨。通过采用一个敏感的荧光测定PPi和校正PPi的水解在孵育过程中,我们检测到PPi释放所有正常哺乳动物软骨研究。通过糖醛酸的产生来测定兔和犬软骨每mg湿重的PPi释放量。半月板纤维软骨是焦磷酸钙沉积最常见的部位,也是PPi的来源。
Calcium pyrophosphate dihydrate crystals are found most frequently in fibrocartilaginous tissue and to a lesser extent in hyaline articular cartilage. Previous investigators found that pyrophosphate (PPi) was released into the medium by immature rabbit hyaline cartilage and osteoarthritic human cartilage in organ culture but not by normal human or mature rabbit cartilage. By employing a sensitive fluorometric assay for PPi and correcting for hydrolysis of PPi during the incubations, we detected PPi release by all normal mammalian cartilage studied. PPi release per mg wet weight of lapine and canine cartilage was paralleled by uronic acid production. Meniscal fibrocartilage, the most common site of calcium pyrophosphate deposits, also elaborated PPi.
DOI: 10.1002/art.1780220813
发表时间: 1979
影响因子: --
作者:
L. Ryan;F. Kozin;D. Mccarty
通讯作者: D. Mccarty
DOI: --
发表时间: 1974
期刊:
影响因子: --
作者:
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通讯作者: Mccarty Dj
DOI: --
发表时间: 1971
期刊: Journal of Laboratory and Clinical Medicine
影响因子: --
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通讯作者: E. Paloyan
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发表时间: 1975
期刊: The Journal of clinical investigation
影响因子: --
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