Processing of pre-microRNAs by the Dicer-1-Loquacious complex in Drosophila cells.
Processing of pre-microRNAs by the Dicer-1-Loquacious complex in Drosophila cells.
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DOI：
10.1371/journal.pbio.0030235
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发表时间：
2005-07
期刊：
影响因子：
9.8
通讯作者：
Siomi MC
中科院分区：
文献类型：
--
作者：
Saito K;Ishizuka A;Siomi H;Siomi MC
microRNA（miRNAs）是一个由21- 22个核苷酸组成的非编码RNA大家族，其在特定位点与靶mRNA相互作用以诱导信息切割或抑制翻译。miRNA以逐步过程从初级miRNA（pri-miRNA）转录物中切除。细胞核中的Drosha-Pasha/DGCR 8复合物切割pri-miRNA以释放发夹形前体miRNA（pre-miRNA）。然后这些前体miRNA被输出到细胞质中，并被Dicer进一步加工成成熟的miRNA。在这里，我们表明，果蝇Dicer-1与Loquacious，一个双链RNA结合结构域蛋白相互作用。Loquacious的消耗导致前体miRNA在果蝇S2细胞中积累，Dicer-1的消耗也是如此。免疫亲和纯化实验表明，Loquacious与Dicer-1沿着存在于功能性pre-miRNA加工复合物中，并刺激和指导特异性pre-miRNA加工活性。这些结果支持了一个模型，其中Loquacious介导miRNA生物合成，从而介导由miRNA调控的基因表达。这篇论文和Förstemann等人的一篇随附论文鉴定了Loquacious，它编码一种双链RNA结合结构域蛋白，并在microRNA的加工中与Dicer-1合作。
microRNAs (miRNAs) are a large family of 21- to 22-nucleotide non-coding RNAs that interact with target mRNAs at specific sites to induce cleavage of the message or inhibit translation. miRNAs are excised in a stepwise process from primary miRNA (pri-miRNA) transcripts. The Drosha-Pasha/DGCR8 complex in the nucleus cleaves pri-miRNAs to release hairpin-shaped precursor miRNAs (pre-miRNAs). These pre-miRNAs are then exported to the cytoplasm and further processed by Dicer to mature miRNAs. Here we show that Drosophila Dicer-1 interacts with Loquacious, a double-stranded RNA-binding domain protein. Depletion of Loquacious results in pre-miRNA accumulation in Drosophila S2 cells, as is the case for depletion of Dicer-1. Immuno-affinity purification experiments revealed that along with Dicer-1, Loquacious resides in a functional pre-miRNA processing complex, and stimulates and directs the specific pre-miRNA processing activity. These results support a model in which Loquacious mediates miRNA biogenesis and, thereby, the expression of genes regulated by miRNAs. This and an accompanying paper by Förstemann et al. identify Loquacious, which encodes a double-stranded RNA binding domain protein, and partners with Dicer-1 in the processing of microRNAs.
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DOI：
10.1073/pnas.110149597
发表时间：
2000-06-06
影响因子：
11.1
作者：
Clemens, JC;Worby, CA;Dixon, JE
通讯作者：
Dixon, JE
影响因子：
64.8
作者：
Denli, AM;Tops, BBJ;Hannon, GJ
通讯作者：
Hannon, GJ
影响因子：
64.5
作者：
Grishok, A;Pasquinelli, AE;Mello, CC
通讯作者：
Mello, CC
影响因子：
4.5
作者：
Djikeng, A;Shi, HF;Ullu, E
通讯作者：
Ullu, E
影响因子：
64.8
作者：
Baulcombe, D
通讯作者：
Baulcombe, D