Rho-kinase directs Bazooka/Par-3 planar polarity during Drosophila axis elongation.
Rho-kinase directs Bazooka/Par-3 planar polarity during Drosophila axis elongation.
复制标题
Rho-kinase在果蝇轴伸长期间指导火箭筒/PAR-3平面极性。
DOI：
10.1016/j.devcel.2010.08.011
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发表时间：
2010-09-14
影响因子：
11.8
通讯作者：
Zallen, Jennifer A.
中科院分区：
文献类型：
--
作者：
Simoes, Sergio de Matos;Blankenship, J. Todd;Weitz, Ori;Farrell, Dene L.;Tamada, Masako;Fernandez-Gonzalez, Rodrigo;Zallen, Jennifer A.
细胞重排通过空间调节细胞形状和粘附的变化来塑造果蝇胚胎。我们发现，Bazooka/Par-3（巴兹）是所需的肌球蛋白II和adherens连接蛋白的平面极化分布和极化的插层行为被破坏巴兹突变体。肌球蛋白II激活剂Rho-激酶以与Baz互补的模式不对称地富集在闰细胞的前后边界。Rho-激酶的缺失导致Baz结构域的扩展，并且激活的Rho-激酶足以将Baz从皮质中排除。Baz的平面极化分布需要其C-末端结构域。Rho激酶可以磷酸化该结构域，并抑制其与磷脂酰肌醇膜脂质的相互作用，这表明Rho激酶可以调节Baz与细胞皮质的联系的机制。这些结果表明，Rho-激酶通过靶向Baz/Par-3和肌球蛋白II互补皮质结构域在平面极性中起指导作用。
Cell rearrangements shape the Drosophila embryo through spatially regulated changes in cell shape and adhesion. We show that Bazooka/Par-3 (Baz) is required for the planar polarized distribution of myosin II and adherens junction proteins and polarized intercalary behavior is disrupted in baz mutants. The myosin II activator Rho-kinase is asymmetrically enriched at anterior and posterior borders of intercalating cells in a pattern complementary to Baz. Loss of Rho-kinase results in expansion of the Baz domain and activated Rho-kinase is sufficient to exclude Baz from the cortex. The planar polarized distribution of Baz requires its C-terminal domain. Rho-kinase can phosphorylate this domain and inhibit its interaction with phosphoinositide membrane lipids, suggesting a mechanism by which Rho-kinase could regulate Baz association with the cell cortex. These results demonstrate that Rho-kinase plays an instructive role in planar polarity by targeting Baz/Par-3 and myosin II to complementary cortical domains.
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