An intron 9 containing splice variant of PAX2.
An intron 9 containing splice variant of PAX2.
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DOI：
10.1186/1479-5876-7-36
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发表时间：
2009-05-25
影响因子：
7.4
通讯作者：
Keilholz U
中科院分区：
文献类型：
--
作者：
Busse A;Rietz A;Schwartz S;Thiel E;Keilholz U
PAX 2是一种转录因子，在胚胎发生发育中起重要作用。然而，PAX 2表达经常在负责癌细胞生长和存活的瘤形成中被鉴定。由于外显子6、外显子10和外显子12的选择性剪接，迄今为止描述了PAX 2的四种同种型。通过定量RT-PCR分析肿瘤B细胞和实体瘤细胞系以及原发性肿瘤样品中含有PAX 2剪接变体的内含子9的表达。PAX 2蛋白通过蛋白质印迹在细胞系的子集中检测。所有14个淋巴瘤细胞系均表达一种未描述的PAX 2剪接变体，该变体包含整个内含子9序列和外显子10序列。这种剪接变体也可以在35种实体瘤细胞系、白血病和淋巴瘤以及结肠癌和黑色素瘤患者样品和健康供体的血液样品中检测到。蛋白质印迹分析证实了这种新的剪接变体在蛋白质水平上的表达。我们在B细胞瘤形成和实体瘤的mRNA和蛋白质水平上发现了一个先前未描述的PAX 2内含子9和外显子10的剪接变体。
PAX2 is a transcription factor with an important role in embryogenic development. However, PAX2 expression was frequently identified in neoplasia responsible for the growth and survival of cancer cells. Due to alternative splicing of exon 6, exon 10 and exon 12 four isoforms of PAX2 are described so far. The expression of an intron 9 containing PAX2 splice variant was analyzed in neoplastic B cell and solid tumor cell lines as well as in primary tumor samples by quantitative RT-PCR. PAX2 proteins were detected by Western Blot in a subset of cell lines. All 14 lymphoma cell lines expressed an undescribed PAX2 splice variant containing the entire intron 9 sequence and the exon 10 sequence. This splice variant could also be detected in 35 solid tumor cell lines, in leukemia and lymphoma as well as in colon carcinoma and melanoma patient samples and in blood samples of healthy donors. Expression of this new splice variant on protein level was verified by Western Blot analysis. We discovered a previously undescribed intron 9 and exon 10 containing splice variant of PAX2 in B-cell neoplasia and in solid tumors on mRNA and protein level.
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影响因子：
8
作者：
Muratovska, A;Zhou, CM;Eccles, MR
通讯作者：
Eccles, MR
影响因子：
3.5
作者：
Ozcan, Ayhan;Zhai, Jim;Truong, Luan D.
通讯作者：
Truong, Luan D.
PAX2致癌基因对前列腺癌中宿主防御肽人β防御的表达负面调节。
影响因子：
3.6
作者：
Bose SK;Gibson W;Bullard RS;Donald CD
通讯作者：
Donald CD
DOI：
10.1073/pnas.89.4.1179
发表时间：
1992-02-15
影响因子：
11.1
作者：
DRESSLER, GR;DOUGLASS, EC
通讯作者：
DOUGLASS, EC
影响因子：
8
作者：
Silberstein, GB;Dressler, GR;Van Horn, K
通讯作者：
Van Horn, K