Mutation of hilD in a Salmonella Derby lineage linked to swine adaptation and reduced risk to human health.
Mutation of hilD in a Salmonella Derby lineage linked to swine adaptation and reduced risk to human health.
复制标题
德比沙门氏菌谱系中hilD的突变与猪的适应性有关，并降低了对人类健康的风险。
DOI：
10.1038/s41598-020-78443-7
复制
发表时间：
2020-12-09
影响因子：
4.6
通讯作者：
Casadei G
中科院分区：
文献类型：
--
作者：
Tambassi M;Berni M;Bracchi C;Scaltriti E;Morganti M;Bolzoni L;Tanner JR;Thilliez G;Kingsley RA;Pongolini S;Casadei G
肠道沙门氏菌变种表现出不同的宿主适应、感染结果以及相关的食品安全风险。对人类和猪中肠沙门氏菌血清型德比 (S. Derby) 亚型分布的分析确定了具有独特 PFGE 谱的分离株，这些分离株在人类感染中明显不足，这与宿主对猪的进一步适应一致。在这里，我们表明，具有这种 PFGE 谱的分离株在 S. Derby 内形成了一个独特的系统发育亚支，并表现出对人上皮细胞的侵袭显着减少，而对猪上皮细胞的侵袭则相对较小。 hilD 中的一个错义突变编码沙门氏菌致病岛 1 (SPI-1) 的主调节因子，存在于适应谱系中。错义突变导致 HilD 功能丧失，从而减少了对人类上皮细胞的侵袭。突变对与猪细胞相互作用的影响相对较小，这与这种病原体-宿主组合的另一种入侵机制一致。
Salmonella enterica variants exhibit diverse host adaptation, outcome of infection, and associated risk to food safety. Analysis of the distribution of Salmonella enterica serovar Derby (S. Derby) subtypes in human and swine identified isolates with a distinct PFGE profile that were significantly under-represented in human infections, consistent with further host adaptation to swine. Here we show that isolates with this PFGE profile form a distinct phylogenetic sub-clade within S. Derby and exhibit a profound reduction in invasion of human epithelial cells, and a relatively small reduction in swine epithelial cells. A single missense mutation in hilD, that encodes the master-regulator of the Salmonella Pathogenicity Island 1 (SPI-1), was present in the adapted lineage. The missense mutation resulted in a loss of function of HilD that accounted for reduced invasion in human epithelial cells. The relatively small impact of the mutation on interaction with swine cells was consistent with an alternative mechanism of invasion in this pathogen-host combination.
登录
查看更多内容
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
3.6
作者：
Hisert, KB;MacCoss, M;Nathan, C
通讯作者：
Nathan, C
影响因子：
3.6
作者：
Darwin, KH;Miller, VL
通讯作者：
Miller, VL
影响因子：
3.7
作者：
Hayward, Matthew R.;AbuOun, Manal;Woodward, Martin J.
通讯作者：
Woodward, Martin J.
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL