Caste- and development-associated gene expression in a lower termite.
Caste- and development-associated gene expression in a lower termite.
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DOI：
10.1186/gb-2003-4-10-r62
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发表时间：
2003
期刊：
影响因子：
12.3
通讯作者：
Bennett GW
中科院分区：
文献类型：
--
作者：
Scharf ME;Wu-Scharf D;Pittendrigh BR;Bennett GW
社会性昆虫，如白蚁，表现出戏剧性的多型性（在一个物种的基础上，差异基因表达的多种形式的出现），无论是与种姓分化和分化后的种姓之间。我们已经使用cDNA宏阵列比较多型种姓和中间发育阶段的白蚁Reticulitermes flavipes之间的基因表达。社会性昆虫，如白蚁，表现出戏剧性的多型性（在一个物种的基础上，差异基因表达的多种形式的出现），无论是与种姓分化和分化后的种姓之间。我们已经使用cDNA宏阵列比较多型种姓和中间发育阶段的白蚁Reticulitermes flavipes之间的基因表达。我们确定了差异表达的基因从九个个体发育类别。定量PCR被用来量化种姓之间和中间发育阶段之间的基因表达的精确差异。我们发现工人和若虫偏向表达的成绩单编码白蚁和内共生体纤维素酶; presoldier偏向表达的成绩单编码的存储/存储结合蛋白卵黄蛋白原;和presoldier偏向表达的基因成绩单编码两个转录/翻译因子，两个信号转导因子和四个细胞骨架/肌肉蛋白。这两个转录/翻译因子与果蝇的bicaudal和bric-a-brac发育基因具有显著的同源性。我们的研究结果表明，差异表达的监管，结构和酶编码基因与白蚁种姓和他们的发展前体阶段。它们还首次揭示了昆虫内共生体纤维素酶基因的表达如何与宿主的种姓相关。这些发现揭示了与白蚁生物学，多型性，种姓分化和发展相关的分子过程，并突出了白蚁，其他昆虫和高等动物之间的发展主题的潜在有趣的变化。
Social insects such as termites express dramatic polyphenism (the occurrence of multiple forms in a species on the basis of differential gene expression) both in association with caste differentiation and between castes after differentiation. We have used cDNA macroarrays to compare gene expression between polyphenic castes and intermediary developmental stages of the termite Reticulitermes flavipes. Social insects such as termites express dramatic polyphenism (the occurrence of multiple forms in a species on the basis of differential gene expression) both in association with caste differentiation and between castes after differentiation. We have used cDNA macroarrays to compare gene expression between polyphenic castes and intermediary developmental stages of the termite Reticulitermes flavipes. We identified differentially expressed genes from nine ontogenic categories. Quantitative PCR was used to quantify precise differences in gene expression between castes and between intermediary developmental stages. We found worker and nymph-biased expression of transcripts encoding termite and endosymbiont cellulases; presoldier-biased expression of transcripts encoding the storage/hormone-binding protein vitellogenin; and soldier-biased expression of gene transcripts encoding two transcription/translation factors, two signal transduction factors and four cytoskeletal/muscle proteins. The two transcription/translation factors showed significant homology to the bicaudal and bric-a-brac developmental genes of Drosophila. Our results show differential expression of regulatory, structural and enzyme-coding genes in association with termite castes and their developmental precursor stages. They also provide the first glimpse into how insect endosymbiont cellulase gene expression can vary in association with the caste of a host. These findings shed light on molecular processes associated with termite biology, polyphenism, caste differentiation and development and highlight potentially interesting variations in developmental themes between termites, other insects, and higher animals.
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影响因子：
4.8
作者：
Lehmann, M;Siegmund, T;Korge, G
通讯作者：
Korge, G
影响因子：
56.9
作者：
Abouheif, E;Wray, GA
通讯作者：
Wray, GA
DOI：
10.1073/pnas.96.10.5575
发表时间：
1999-05-11
影响因子：
11.1
作者：
Evans, JD;Wheeler, DE
通讯作者：
Wheeler, DE
影响因子：
3.8
作者：
Kawasaki, H;Sugaya, K;Mita, K
通讯作者：
Mita, K
影响因子：
3.8
作者：
Nakashima, K;Watanabe, H;Azuma, JI
通讯作者：
Azuma, JI