The requirement for calcification differs between ecologically important coccolithophore species.
The requirement for calcification differs between ecologically important coccolithophore species.
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DOI：
10.1111/nph.15272
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发表时间：
2018-10
期刊：
影响因子：
--
通讯作者：
Wheeler GL
中科院分区：
文献类型：
--
作者：
Walker CE;Taylor AR;Langer G;Durak GM;Heath S;Probert I;Tyrrell T;Brownlee C;Wheeler GL
颗石藻是一种全球性分布的单细胞海藻，其特征是表面覆盖有方解石颗石。颗石藻的钙化作用对全球地球化学循环有重要贡献。然而，对钙化的生理要求仍然知之甚少，因为一些常用的模式物种的非钙化菌株，如Emiliania huxleyi，在实验室培养中正常生长。为了确定不同颗石藻种对钙化的要求是否不同，我们利用多种独立的方法来破坏两种重要的颗石藻种的钙化：E。huxleyi和Coccolithus braarudii。我们研究了它们的生理反应，并使用延时成像来可视化单个细胞中的钙化和细胞分裂过程。破坏钙化导致布拉氏梭菌的主要生长缺陷，但在赫胥黎氏真蜱中没有。我们没有发现证据表明钙化支持C. braarudii的光合作用，但表明无法维持完整的球珠导致细胞周期停滞。我们发现C. braarudii与E. huxleyi非常不同，因为它表现出对钙化的专性要求。确定C. braarudii的生长缺陷导致的破坏的coccosphere可能是重要的，在考虑他们对未来的海洋碳酸盐化学变化的反应。另见https://doi.org/10.1111/nph.15409对这篇文章的评论。
Coccolithophores are globally distributed unicellular marine algae that are characterized by their covering of calcite coccoliths. Calcification by coccolithophores contributes significantly to global biogeochemical cycles. However, the physiological requirement for calcification remains poorly understood as non‐calcifying strains of some commonly used model species, such as Emiliania huxleyi, grow normally in laboratory culture. To determine whether the requirement for calcification differs between coccolithophore species, we utilized multiple independent methodologies to disrupt calcification in two important species of coccolithophore: E. huxleyi and Coccolithus braarudii. We investigated their physiological response and used time‐lapse imaging to visualize the processes of calcification and cell division in individual cells. Disruption of calcification resulted in major growth defects in C. braarudii, but not in E. huxleyi. We found no evidence that calcification supports photosynthesis in C. braarudii, but showed that an inability to maintain an intact coccosphere results in cell cycle arrest. We found that C. braarudii is very different from E. huxleyi as it exhibits an obligate requirement for calcification. The identification of a growth defect in C. braarudii resulting from disruption of the coccosphere may be important in considering their response to future changes in ocean carbonate chemistry. See also the Commentary on this article by https://doi.org/10.1111/nph.15409.
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影响因子：
3.2
作者：
CORPE, WA
通讯作者：
CORPE, WA
DOI：
10.3891/acta.chem.scand.30b-0562
发表时间：
1976-01-01
期刊：
ACTA CHEMICA SCANDINAVICA SERIES B-ORGANIC CHEMISTRY AND BIOCHEMISTRY
影响因子：
--
作者：
HAUG, A
通讯作者：
HAUG, A
影响因子：
2.9
作者：
HARRISON, PJ;WATERS, RE;TAYLOR, FJR
通讯作者：
TAYLOR, FJR
影响因子：
7.4
作者：
Domozych, David S.;Sorensen, Iben;Rose, Jocelyn K. C.
通讯作者：
Rose, Jocelyn K. C.
影响因子：
2.8
作者：
GUILLARD, RR;RYTHER, JH
通讯作者：
RYTHER, JH