Quantitative image analysis reveals distinct structural transitions during aging in Caenorhabditis elegans tissues.
Quantitative image analysis reveals distinct structural transitions during aging in Caenorhabditis elegans tissues.
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DOI：
10.1371/journal.pone.0002821
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发表时间：
2008-07-30
期刊：
影响因子：
3.7
通讯作者：
Wolkow, Catherine A.
中科院分区：
文献类型：
--
作者：
Johnston, Josiah;Iser, Wendy B.;Chow, David K.;Goldberg, Ilya G.;Wolkow, Catherine A.
衰老与许多身体系统的功能和结构下降有关，即使没有潜在的疾病。特别是，骨骼肌在衰老过程中经历严重衰退，这种现象称为肌肉减少症。尽管肌肉减少症的发病率和严重程度很高，但对其影响因素和发展知之甚少。许多研究集中在与衰老相关的组织衰退的功能方面，而结构细节仍然研究不足。用于量化结构变化的传统方法以离散间隔评估个体标记物。这些方法不足以应对与衰老相关的复杂变化。另一种方法是考虑整体形态学而不是特定标志物的变化。我们已经使用这种方法来定量跟踪组织结构在成年和衰老的C。线虫的咽神经肌肉摄食器官使用模式识别来分析年龄分组的咽部图像，我们确定了不同形态之间的离散逐步过渡。形态学状态转换保持在突变体咽神经传递缺陷，虽然改变了过渡的步伐。咽功能的纵向测量确定了中年咽形态和以后年龄咽功能之间的预测关系。这些研究首次表明，成人组织经历了不同的结构转变，反映了发育后的事件。这些结构变化的基础过程可能导致衰老过程中疾病风险的增加，并且可能是改变衰老速率的因素的目标。这项工作进一步表明，图像系列的模式分析提供了一种新的和普遍可用的方法，用于量化形态学变化和识别结构生物标志物。
Aging is associated with functional and structural declines in many body systems, even in the absence of underlying disease. In particular, skeletal muscles experience severe declines during aging, a phenomenon termed sarcopenia. Despite the high incidence and severity of sarcopenia, little is known about contributing factors and development. Many studies focus on functional aspects of aging-related tissue decline, while structural details remain understudied. Traditional approaches for quantifying structural changes have assessed individual markers at discrete intervals. Such approaches are inadequate for the complex changes associated with aging. An alternative is to consider changes in overall morphology rather than in specific markers. We have used this approach to quantitatively track tissue architecture during adulthood and aging in the C. elegans pharynx, the neuromuscular feeding organ. Using pattern recognition to analyze aged-grouped pharynx images, we identified discrete step-wise transitions between distinct morphologies. The morphology state transitions were maintained in mutants with pharynx neurotransmission defects, although the pace of the transitions was altered. Longitudinal measurements of pharynx function identified a predictive relationship between mid-life pharynx morphology and function at later ages. These studies demonstrate for the first time that adult tissues undergo distinct structural transitions reflecting postdevelopmental events. The processes that underlie these architectural changes may contribute to increased disease risk during aging, and may be targets for factors that alter the aging rate. This work further demonstrates that pattern analysis of an image series offers a novel and generally accessible approach for quantifying morphological changes and identifying structural biomarkers.
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DOI：
10.1073/pnas.0400848101
发表时间：
2004-05-25
影响因子：
11.1
作者：
Huang, C;Xiong, CJ;Kornfeld, K
通讯作者：
Kornfeld, K
影响因子：
64.8
作者：
Herndon, LA;Schmeissner, PJ;Driscoll, M
通讯作者：
Driscoll, M
DOI：
10.1093/gerona/61.1.72
发表时间：
2006-01-01
影响因子：
5.1
作者：
Newman, AB;Kupelian, V;Harris, TB
通讯作者：
Harris, TB
影响因子：
--
作者：
Helfrich, Yolanda R.;Yu, Le;King, Anya
通讯作者：
King, Anya
影响因子：
56.9
作者：
de Fockert, JW;Rees, G;Lavie, N
通讯作者：
Lavie, N