Cloning, expression, and characterization of thermotolerant manganese superoxide dismutase from Bacillus sp. MHS47.
Cloning, expression, and characterization of thermotolerant manganese superoxide dismutase from Bacillus sp. MHS47.
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DOI：
10.3390/ijms12010844
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发表时间：
2011-01-24
影响因子：
5.6
通讯作者：
Chansiri K
中科院分区：
文献类型：
--
作者：
Areekit S;Kanjanavas P;Khawsak P;Pakpitchareon A;Potivejkul K;Chansiri G;Chansiri K
来自耐热芽孢杆菌属的超氧化物歧化酶基因。 MHS47 (MnSOD47) 被克隆、测序和表达。该基因开放阅读框长612 bp，推导203个氨基酸，与苏云金芽孢杆菌（登录号：EEN01322）、炭疽芽孢杆菌（登录号：NP_846724）、蜡样芽孢杆菌（登录号：ZP_04187911）、维恩斯特凡芽孢杆菌（登录号：ZP_04187911）的氨基酸序列具有较高的同源性。号 YP_001646918）和假蕈状芽孢杆菌。保守的锰结合位点（H28、H83、D165 和 H169）表明 MnSOD47 具有锰超氧化物歧化酶 (MnSOD) 的特定特征。 MnSOD47表达的酶的分子量约为22.65 kDa，比活性为3537.75 U/mg。该酶在 pH 范围 7-8.5 范围内活跃，最适 pH 值为 7.5，温度范围为 30-45 °C，最适温度为 37 °C。抑制剂和金属离子测试表明，叠氮化钠抑制酶活性，但过氧化氢或氰化钾不抑制酶活性。这些数据应该有利于未来对其他微生物中 MnSOD 的研究以及 MnSOD47 的生物技术生产，并且还可以用于开发用于检测抗氧化剂和自由基活性的生物传感器。将来，这些基础知识可以应用于人类癌症风险的检测和治疗。
A superoxide dismutase gene from thermotolerant Bacillus sp. MHS47 (MnSOD47) was cloned, sequenced, and expressed. The gene has an open reading frame of 612 bp, corresponding to 203 deduced amino acids, with high homology to the amino acid sequences of B. thuringiensis (accession no. EEN01322), B. anthracis (accession no. NP_846724), B. cereus (accession no. ZP_04187911), B. weihenstephanensis (accession no. YP_001646918), and B. pseudomycoides. The conserved manganese-binding sites (H28, H83, D165, and H169) show that MnSOD47 has the specific characteristics of the manganese superoxide dismutase (MnSOD) enzymes. MnSOD47 expressed an enzyme with a molecular weight of approximately 22.65 kDa and a specific activity of 3537.75 U/mg. The enzyme is active in the pH range 7–8.5, with an optimum pH of 7.5, and at temperatures in the range 30–45 °C, with an optimum temperature of 37 °C. Tests of inhibitors and metal ions indicated that the enzyme activity is inhibited by sodium azide, but not by hydrogen peroxide or potassium cyanide. These data should benefit future studies of MnSODs in other microorganisms and the biotechnological production of MnSOD47, and could also be used to develop a biosensor for the detection of antioxidants and free radical activity. In the future, this basic knowledge could be applicable to the detection of cancer risks in humans and therapeutic treatments.
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影响因子：
4.3
作者：
Emregül, E
通讯作者：
Emregül, E
影响因子：
2.9
作者：
Li, DC;Gao, J;Lu, J
通讯作者：
Lu, J
影响因子：
3.5
作者：
PARKER, MW;BLAKE, CCF
通讯作者：
BLAKE, CCF
影响因子：
1.6
作者：
Ken, CF;Lee, CC;Lin, CT
通讯作者：
Lin, CT
DOI：
10.1016/s0922-338x(98)80028-7
发表时间：
1998-01-01
期刊：
JOURNAL OF FERMENTATION AND BIOENGINEERING
影响因子：
--
作者：
Motoshima, H;Minagawa, E;Kaminogawa, S
通讯作者：
Kaminogawa, S