Expression of fatty acid synthesis genes and fatty acid accumulation in haematococcus pluvialis under different stressors.
Expression of fatty acid synthesis genes and fatty acid accumulation in haematococcus pluvialis under different stressors.
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DOI：
10.1186/1754-6834-5-18
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发表时间：
2012-03-26
影响因子：
6.3
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Lei A;Chen H;Shen G;Hu Z;Chen L;Wang J
过去几年，生物燃料一直是全球深入研究的焦点。基于藻类代谢工程的第四代生物燃料生产（藻类到生物燃料）的发展仍处于起步阶段，主要障碍之一是我们对微藻生长、代谢和生物燃料生产缺乏了解。尽管脂肪酸（FA）生物合成途径基因已全部克隆，并在一些高等植物中建立了生物合成途径，但其在微藻中调控的分子机制还远未阐明。我们克隆了雨生红球藻（一种作为潜在生物柴油原料的绿色微藻）FA生物合成的主要关键基因，并研究了在不同胁迫处理（如氮消耗、盐度、高温或低温）下它们的表达交替与GC-MS检测到的FA组成和含量之间的相关性。我们的结果表明，高温、高盐和缺氮处理对微藻 FA 合成具有显着的促进作用，而 FA 品质没有太大变化。相关分析表明，酰基载体蛋白（ACP）、3-酮酰基-ACP-合酶（KAS）和酰基-ACP硫酯酶（FATA）基因表达与单不饱和FA（MUFA）合成和多不饱和FA（PUFA）合成具有显着相关性。我们提出雨生红藻中的 ACP、KAS 和 FATA 可能在 FA 合成中发挥重要作用，并且可能是限速基因，可能可以对其进行修改，以用于代谢工程的进一步研究，以提高微藻生物燃料的质量和产量。
Biofuel has been the focus of intensive global research over the past few years. The development of 4th generation biofuel production (algae-to-biofuels) based on metabolic engineering of algae is still in its infancy, one of the main barriers is our lacking of understanding of microalgal growth, metabolism and biofuel production. Although fatty acid (FA) biosynthesis pathway genes have been all cloned and biosynthesis pathway was built up in some higher plants, the molecular mechanism for its regulation in microalgae is far away from elucidation. We cloned main key genes for FA biosynthesis in Haematococcus pluvialis, a green microalga as a potential biodiesel feedstock, and investigated the correlations between their expression alternation and FA composition and content detected by GC-MS under different stress treatments, such as nitrogen depletion, salinity, high or low temperature. Our results showed that high temperature, high salinity, and nitrogen depletion treatments played significant roles in promoting microalgal FA synthesis, while FA qualities were not changed much. Correlation analysis showed that acyl carrier protein (ACP), 3-ketoacyl-ACP-synthase (KAS), and acyl-ACP thioesterase (FATA) gene expression had significant correlations with monounsaturated FA (MUFA) synthesis and polyunsaturated FA (PUFA) synthesis. We proposed that ACP, KAS, and FATA in H. pluvialis may play an important role in FA synthesis and may be rate limiting genes, which probably could be modified for the further study of metabolic engineering to improve microalgal biofuel quality and production.
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影响因子：
6.3
作者：
Pereira H;Barreira L;Mozes A;Florindo C;Polo C;Duarte CV;Custódio L;Varela J
通讯作者：
Varela J
影响因子：
6
作者：
Azam, MM;Waris, A;Nahar, NM
通讯作者：
Nahar, NM
影响因子：
11.4
作者：
Mutanda, T.;Ramesh, D.;Bux, F.
通讯作者：
Bux, F.
影响因子：
11.4
作者：
Jesus Ramos, Maria;Maria Fernandez, Carmen;Perez, Angel
通讯作者：
Perez, Angel
影响因子：
2
作者：
KRISNANGKURA, K
通讯作者：
KRISNANGKURA, K