Synaptic remodeling in mouse motor cortex after spinal cord injury.
Synaptic remodeling in mouse motor cortex after spinal cord injury.
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脊髓损伤后小鼠运动皮层的突触重塑。
DOI：
10.4103/1673-5374.295346
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发表时间：
2021-04
影响因子：
6.1
通讯作者：
Chen JY
中科院分区：
文献类型：
--
作者：
Zhang KX;Zhao JJ;Chai W;Chen JY
脊髓损伤极大地阻碍了中枢神经系统和周围神经系统之间的信息交换。运动皮层突触的最终命运尚未得到充分研究。为了探讨脊髓损伤后运动皮层的突触重组，我们建立了T12脊髓半切片的小鼠模型，然后在体内监测运动皮层后肢区域锥体神经元的突触后树突棘和突触前轴突。我们的结果表明，脊髓半切导致运动皮层双侧树突棘的重塑，并且主要重塑区域随着时间的推移而变化。它使以前稳定的刺变得不稳定，并消除了刺更不可能重新出现。对侧运动皮层的新棘明显增加。然而，新刺的存活率很低，这表明新刺仍然脆弱。观察突触前轴突布顿没有发现显着变化。这些结果表明脊髓半切断后运动皮层中存在突触重塑，并且脊髓半切断影响突触后树突棘而不是突触前轴突。本研究于2015年4月16日获得中国人民解放军总医院伦理委员会批准（批准号：201504168S）。
Spinal cord injury dramatically blocks information exchange between the central nervous system and the peripheral nervous system. The resulting fate of synapses in the motor cortex has not been well studied. To explore synaptic reorganization in the motor cortex after spinal cord injury, we established mouse models of T12 spinal cord hemi-section and then monitored the postsynaptic dendritic spines and presynaptic axonal boutons of pyramidal neurons in the hindlimb area of the motor cortex in vivo. Our results showed that spinal cord hemi-section led to the remodeling of dendritic spines bilaterally in the motor cortex and the main remodeling regions changed over time. It made previously stable spines unstable and eliminated spines more unlikely to be re-emerged. There was a significant increase in new spines in the contralateral motor cortex. However, the low survival rate of the new spines demonstrated that new spines were still fragile. Observation of presynaptic axonal boutons found no significant change. These results suggest the existence of synapse remodeling in motor cortex after spinal cord hemi-section and that spinal cord hemi-section affected postsynaptic dendritic spines rather than presynaptic axonal boutons. This study was approved by the Ethics Committee of Chinese PLA General Hospital, China (approval No. 201504168S) on April 16, 2015.
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