Epithelial proinflammatory response and curcumin-mediated protection from staphylococcal toxic shock syndrome toxin-1.
Epithelial proinflammatory response and curcumin-mediated protection from staphylococcal toxic shock syndrome toxin-1.
复制标题
DOI：
10.1371/journal.pone.0032813
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Peterson ML
中科院分区：
文献类型：
--
作者：
Schaefers MM;Breshears LM;Anderson MJ;Lin YC;Grill AE;Panyam J;Southern PJ;Schlievert PM;Peterson ML
Staphylococcus aureus initiates infections and produces virulence factors, including superantigens (SAgs), at mucosal surfaces. The SAg, Toxic Shock Syndrome Toxin-1 (TSST-1) induces cytokine secretion from epithelial cells, antigen presenting cells (APCs) and T lymphocytes, and causes toxic shock syndrome (TSS). This study investigated the mechanism of TSST-1-induced secretion of proinflammatory cytokines from human vaginal epithelial cells (HVECs) and determined if curcumin, an anti-inflammatory agent, could reduce TSST-1-mediated pathology in a rabbit vaginal model of TSS. TSST-1 caused a significant increase in NF-κB-dependent transcription in HVECs that was associated with increased expression of TNF- α, MIP-3α, IL-6 and IL-8. Curcumin, an antagonist of NF-κB-dependent transcription, inhibited IL-8 production from ex vivo porcine vaginal explants at nontoxic doses. In a rabbit model of TSS, co-administration of curcumin with TSST-1 intravaginally reduced lethality by 60% relative to 100% lethality in rabbits receiving TSST-1 alone. In addition, TNF-α was undetectable from serum or vaginal tissue of curcumin treated rabbits that survived. These data suggest that the inflammatory response induced at the mucosal surface by TSST-1 is NF-κB dependent. In addition, the ability of curcumin to prevent TSS in vivo by co-administration with TSST-1 intravaginally suggests that the vaginal mucosal proinflammatory response to TSST-1 is important in the progression of mTSS.
登录
查看更多内容
细胞素蛋白增强分层粘膜的超抗原渗透。
DOI：
10.4049/jimmunol.0803283
发表时间：
2009-02-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Brosnahan AJ;Mantz MJ;Squier CA;Peterson ML;Schlievert PM
通讯作者：
Schlievert PM
影响因子：
4.9
作者：
Anderson, Michele J.;Horn, Mary E.;Peterson, Marnie L.
通讯作者：
Peterson, Marnie L.
影响因子：
3
作者：
Bansal, Shruti;Chhibber, Sanjay
通讯作者：
Chhibber, Sanjay
影响因子：
3.1
作者：
Lueer, Sonja;Troller, Rolf;Aebi, Christoph
通讯作者：
Aebi, Christoph
影响因子：
5.8
作者：
O'Brien GJ;Riddell G;Elborn JS;Ennis M;Skibinski G
通讯作者：
Skibinski G