Meta-genomic analysis of toilet waste from long distance flights; a step towards global surveillance of infectious diseases and antimicrobial resistance.
Meta-genomic analysis of toilet waste from long distance flights; a step towards global surveillance of infectious diseases and antimicrobial resistance.
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DOI：
10.1038/srep11444
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发表时间：
2015-07-10
影响因子：
4.6
通讯作者：
Aarestrup FM
中科院分区：
文献类型：
--
作者：
Nordahl Petersen T;Rasmussen S;Hasman H;Carøe C;Bælum J;Schultz AC;Bergmark L;Svendsen CA;Lund O;Sicheritz-Pontén T;Aarestrup FM
世界范围内的人类越来越多地面临传染病和抗菌素耐药性，传播速度更快，出现频率更高。了解它们的发生和在世界范围内的传播对控制疫情和预防流行病很重要。在这里，我们对从三个世界地区的九个城市抵达丹麦哥本哈根的18架国际飞机上的厕所废物进行了鸟枪测序。平均生成18.6 Gb （14.8 ~ 25.7 Gb）的原始Illumina配对末端序列数据，对其进行清洗、修剪，并与细菌和抗菌基因的参考序列数据库进行比对。平均106,839（0.06%）个reads被分配到抗性基因中，其中编码四环素、大环内酯和β -内酰胺抗性基因的基因数量最多。我们发现，与北美相比，来自南亚的飞机携带的编码抗菌素耐药性的基因的丰度和多样性明显更高，包括至关重要的耐药性（如blaCTX-M）。在南亚的样本中也检测到较多的肠沙门氏菌和诺如病毒，而在北美的样本中，难辨梭菌含量最多。我们的研究为全球监测提供了潜在的新策略的第一步，该策略能够同时检测多种威胁人类健康的遗传因子、感染因子和抗性基因。
Human populations worldwide are increasingly confronted with infectious diseases and antimicrobial resistance spreading faster and appearing more frequently. Knowledge regarding their occurrence and worldwide transmission is important to control outbreaks and prevent epidemics. Here, we performed shotgun sequencing of toilet waste from 18 international airplanes arriving in Copenhagen, Denmark, from nine cities in three world regions. An average of 18.6 Gb (14.8 to 25.7 Gb) of raw Illumina paired end sequence data was generated, cleaned, trimmed and mapped against reference sequence databases for bacteria and antimicrobial resistance genes. An average of 106,839 (0.06%) reads were assigned to resistance genes with genes encoding resistance to tetracycline, macrolide and beta-lactam resistance genes as the most abundant in all samples. We found significantly higher abundance and diversity of genes encoding antimicrobial resistance, including critical important resistance (e.g. blaCTX-M) carried on airplanes from South Asia compared to North America. Presence of Salmonella enterica and norovirus were also detected in higher amounts from South Asia, whereas Clostridium difficile was most abundant in samples from North America. Our study provides a first step towards a potential novel strategy for global surveillance enabling simultaneous detection of multiple human health threatening genetic elements, infectious agents and resistance genes.
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影响因子：
11.8
作者：
Aarestrup FM;Brown EW;Detter C;Gerner-Smidt P;Gilmour MW;Harmsen D;Hendriksen RS;Hewson R;Heymann DL;Johansson K;Ijaz K;Keim PS;Koopmans M;Kroneman A;Lo Fo Wong D;Lund O;Palm D;Sawanpanyalert P;Sobel J;Schlundt J
通讯作者：
Schlundt J
影响因子：
--
作者：
Meyer, Matthias;Kircher, Martin
通讯作者：
Kircher, Martin
影响因子：
168.9
作者：
Lozano, Rafael;Naghavi, Mohsen;Murray, Christopher J. L.
通讯作者：
Murray, Christopher J. L.
DOI：
10.1126/science.1245993
发表时间：
2014-10-17
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Carroll SP;Jørgensen PS;Kinnison MT;Bergstrom CT;Denison RF;Gluckman P;Smith TB;Strauss SY;Tabashnik BE
通讯作者：
Tabashnik BE
DOI：
10.1073/pnas.1006219107
发表时间：
2010-12-14
影响因子：
11.1
作者：
Chan, Emily H.;Brewer, Timothy F.;Brownstein, John S.
通讯作者：
Brownstein, John S.