Exploring substrate promiscuity of chlorophenol hydroxylase against biphenyl derivatives
Exploring substrate promiscuity of chlorophenol hydroxylase against biphenyl derivatives
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探索氯酚羟化酶对联苯衍生物的底物混杂性
DOI：
10.1039/c5ra16935f
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发表时间：
2015-10
期刊：
影响因子：
3.9
通讯作者：
Dahai Yu
中科院分区：
文献类型：
--
作者：
Hejun Ren;Qingchao Li;Xuexun Fang;Dahai Yu
2，4-二氯苯酚羟基酶的基因来源于多氯联苯(PCBs)污染土壤的后基因组文库，它对包括氯苯酚(CPS)及其同系物在内的单环芳香污染物具有广泛的活性。在这项研究中，我们打算探索它对芳香族双环化合物，如联苯及其衍生物的活性，这些化合物也是重要的持久性环境污染物。结果表明，该酶对羟基联苯基、卤代联苯、多氯联苯和羟基多氯联苯具有广泛的底物专一性，使其底物混杂程度超出了CPS及其同系物。对这些芳香族双环化合物的酶活性依赖于同系物，联苯衍生物上取代基的位置和类型对该酶的底物优势性有很大影响。在分析了该酶对联苯类化合物的催化活性的基础上，初步提出了该酶在芳环上的催化优势假说。该酶对选定的芳香族污染物具有很高的活性和去除能力，是一种非常有前途的联苯类化合物生物修复催化剂。
A 2,4-dichlorophenol hydroxylase, whose gene was derived from the metagenomic library of polychlorinated biphenyl (PCB)-contaminated soil has been found to exhibit a broad range of activity for single ring aromatic contaminants including chlorophenols (CPs) and their homologues. In this study, we intended to explore its activity to aromatic bicyclic compounds such as biphenyl and its derivatives which are also important persistent environmental contaminants. Results demonstrated that the enzyme exhibited broad substrate specificity to selected biphenyl derivatives including hydroxylated biphenyls, halogenated biphenyls, PCBs and hydroxylated PCBs, which extended its substrate promiscuity apart from CPs and their homologues. The enzymatic activities against these aromatic bicyclic compounds were congener dependent and the position and type of the substituent on biphenyl derivatives greatly affected the substrate priority of this enzyme. The hypothesis of the catalysis preference of the enzyme on the aromatic ring was preliminarily proposed on the basis of the analyses of the enzymatic activities against biphenyl derivatives. The high activity and removal ability of this enzyme against selected aromatic contaminants would make it a very promising catalyst for bioremediation of biphenyl derivatives.
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影响因子：
3.9
作者：
Hejun Ren;Yang Zhan;Xuexun Fang;Dahai Yu
通讯作者：
Hejun Ren;Yang Zhan;Xuexun Fang;Dahai Yu
DOI：
10.1073/pnas.0602398103
发表时间：
2006-06-27
影响因子：
11.1
作者：
Eswaramoorthy, Subramaniam;Bonanno, Jeffrey B.;Swaminathan, Subramanyam
通讯作者：
Swaminathan, Subramanyam
影响因子：
6.6
作者：
Lopez-Iglesias, Maria;Gotor-Fernandez, Vicente
通讯作者：
Gotor-Fernandez, Vicente
影响因子：
11.4
作者：
Aeppli, Christoph;Tysklind, Mats;Gustafsson, Orjan
通讯作者：
Gustafsson, Orjan
影响因子：
13.8
作者：
Copley SD
通讯作者：
Copley SD