Engineering large, anatomically shaped osteochondral constructs with robust interfacial shear properties.
Engineering large, anatomically shaped osteochondral constructs with robust interfacial shear properties.
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DOI：
10.1038/s41536-021-00152-0
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发表时间：
2021-08-06
影响因子：
7.2
通讯作者：
Athanasiou KA
中科院分区：
文献类型：
--
作者：
Brown WE;Huang BJ;Hu JC;Athanasiou KA
尽管涉及底层骨的大面积（>5cm2）关节软骨缺陷普遍存在，但目前的组织工程疗法只能解决小缺陷。组织工程、符合解剖学形状、类似天然的植入物可能会满足对大软骨损伤的现成组织修复疗法的需求。这项研究制造了一种具有平移相关几何形状的骨软骨结构，具有强大的功能特性。无支架的自组装新软骨充当软骨相，多孔羟基磷灰石充当骨软骨结构的骨相。在新软骨成熟的第 4 天（早期）或第 10 天（晚期）组装形状和尺寸与绵羊股骨髁 (31×14 mm) 相同的结构。与后期组装相比，早期骨软骨组装使界面交错深度增加了244%，交错频率增加了438%，界面剪切模量增加了243倍，极限界面剪切强度增加了4.9倍。为了开发一种用于修复覆盖整个髁表面的软骨损伤的生物假体，这项研究产生了一种大型的、符合解剖学形状的骨软骨结构，具有强大的界面机械性能和类似天然的新软骨相互交错。
Despite the prevalence of large (>5 cm2) articular cartilage defects involving underlying bone, current tissue-engineered therapies only address small defects. Tissue-engineered, anatomically shaped, native-like implants may address the need for off-the-shelf, tissue-repairing therapies for large cartilage lesions. This study fabricated an osteochondral construct of translationally relevant geometry with robust functional properties. Scaffold-free, self-assembled neocartilage served as the chondral phase, and porous hydroxyapatite served as the osseous phase of the osteochondral constructs. Constructs in the shape and size of an ovine femoral condyle (31 × 14 mm) were assembled at day 4 (early) or day 10 (late) of neocartilage maturation. Early osteochondral assembly increased the interfacial interdigitation depth by 244%, interdigitation frequency by 438%, interfacial shear modulus by 243-fold, and ultimate interfacial shear strength by 4.9-fold, compared to late assembly. Toward the development of a bioprosthesis for the repair of cartilage lesions encompassing up to an entire condylar surface, this study generated a large, anatomically shaped osteochondral construct with robust interfacial mechanical properties and native-like neocartilage interdigitation.
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