The influence of an aligned nanofibrous topography on human mesenchymal stem cell fibrochondrogenesis.
The influence of an aligned nanofibrous topography on human mesenchymal stem cell fibrochondrogenesis.
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DOI：
10.1016/j.biomaterials.2010.04.036
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发表时间：
2010-08
期刊：
影响因子：
14
通讯作者：
Mauck, Robert L.
中科院分区：
文献类型：
--
作者：
Baker, Brendon M.;Nathan, Ashwin S.;Gee, Albert O.;Mauck, Robert L.
纤维软骨组织在全身许多关节中起着关键的承重作用。由于这些结构经常受到损伤，因此对修复或替换的工程化组织的需求很大。这项研究评估了人骨髓间充质干细胞在纳米纤维微环境中形成具有机械功能的纤维软骨基质的能力。由超细可生物降解聚合物纤维组成的纳米纤维支架复制了天然纤维组织的结构和力学各向异性，并作为一种三维微图案来指导细胞取向和有序基质的形成。从4例骨关节炎(OA)患者中分离出间充质干细胞(MSC)和半月板纤维软骨细胞(FC)，后者已被证明是构建纤维软骨的有效细胞来源。细胞种植的纳米纤维支架在化学定义的介质配方中培养，并在9周内评估机械、生化和组织学特征。令人惊讶的是，与之前对幼年牛细胞的研究相反，与类似培养的供体匹配的FCs相比，成年人MSCs的基质组装显著受阻。与FCS不同的是，MSCs没有增殖，导致结构稀疏定植。与FC构建物相比，MSC种子构建物中基质含量的增加，因此拉伸性能的变化是温和的，即使当归一化到这些构建物中较低的细胞数量时也是如此。为了排除OA来源对MSCs的影响，我们产生了来自健康年轻捐赠者的构建；这些构建成熟时与那些来自OA MSCs的构建没有什么不同。重要的是，当以颗粒形式培养时，MSCs和FCS的基质产生没有差异，这突显了人MSCs对其三维微环境的敏感性。
Fibrocartilaginous tissues serve critical load-bearing functions in numerous joints throughout the body. As these structures are often injured, there exists great demand for engineered tissue for repair or replacement. This study assessed the ability of human marrow-derived MSCs to elaborate a mechanically functional, fibrocartilaginous matrix in a nanofibrous microenvironment. Nanofibrous scaffolds, composed of ultra-fine biodegradable polymer fibers, replicate the structural and mechanical anisotropy of native fibrous tissues and serve as a 3D micro-pattern for directing cell orientation and ordered matrix formation. Mesenchymal stem cells (MSC) were isolated from four osteoarthritic (OA) patients, along with meniscal fibrochondrocytes (FC) which have proven to be a potent cell source for engineering fibrocartilage. Cell-seeded nanofibrous scaffolds were cultured in a chemically-defined medium formulation and mechanical, biochemical, and histological features were evaluated over 9 weeks. Surprisingly, and contrary to previous studies with juvenile bovine cells, matrix assembly by adult human MSCs was dramatically hindered compared to donor-matched FCs cultured similarly. Unlike FCs, MSCs did not proliferate, resulting in sparsely colonized constructs. Increases in matrix content, and therefore changes in tensile properties, were modest in MSC-seeded constructs compared to FC counterparts, even when normalized to the lower cell number in these constructs. To rule out the influence of OA sourcing on MSCs, constructs from healthy young donors were generated; these constructs matured no differently than those formed with OA MSCs. Importantly, there was no difference in matrix production of MSCs and FCs when cultured in pellet form, highlighting the sensitivity of human MSCs to their 3D microenvironment.
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DOI：
10.1089/clo.2008.0070
发表时间：
2009-03-01
期刊：
CLONING AND STEM CELLS
影响因子：
--
作者：
Mrugala, Dominique;Dossat, Nadege;Jorgensen, Christian
通讯作者：
Jorgensen, Christian
影响因子：
1.8
作者：
Farre, Jordi;Roura, Santiago;Bayes-Genis, Antoni
通讯作者：
Bayes-Genis, Antoni
DOI：
10.1016/0304-4165(86)90306-5
发表时间：
1986-09-04
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
FARNDALE, RW;BUTTLE, DJ;BARRETT, AJ
通讯作者：
BARRETT, AJ
影响因子：
2.8
作者：
Nerurkar, Nandan L.;Elliott, Dawn M.;Mauck, Robert L.
通讯作者：
Mauck, Robert L.
影响因子：
14
作者：
Baker, Brendon M.;Mauck, Robert L.
通讯作者：
Mauck, Robert L.