Transposon accumulation at xenobiotic gene family loci in aphids.
Transposon accumulation at xenobiotic gene family loci in aphids.
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DOI：
10.1101/gr.277820.123
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发表时间：
2023-10
期刊：
影响因子：
7
通讯作者：
Hayward, Alex
中科院分区：
文献类型：
--
作者：
Baril, Tobias;Pym, Adam;Bass, Chris;Hayward, Alex
抗性的演变是可持续控制害虫和病原体的一个主要挑战。因此，为了保障健康和粮食生产，需要更深入地了解耐药性进化的进化和基因组机制。一些研究表明转座因子（TEs）参与了昆虫对异源生物的抗性进化。然而，分析通常限于一个昆虫物种和/或一个或几个异生素基因家族（XGF）。我们通过对20个蚜虫基因组进行比较基因组分析，研究了XGF中TE积累的证据，考虑了参与杀虫剂代谢抗性的XGF的主要子集：细胞色素P450，谷胱甘肽S-转移酶，酯酶，UDP-葡萄糖醛酸转移酶和ABC转运蛋白。我们发现，与其他基因相比，TE在XGF上显著富集。XGF显示出与持家基因相似的TE富集水平。但与管家基因不同，XGF在生殖细胞中不组成型表达，支持TE在XGF处的选择性富集，而不是由于染色质可用性而富集。极端TE富集的热点发生在某些XGF周围。我们发现，在蚜虫的农业重要性，特别是丰富的TE细胞色素P450基因与已知的功能，在合成杀虫剂的解毒。我们的研究结果提供的证据支持的一般作用，TE作为一个来源的基因组变异在主机XGF和突出存在相当大的变异性TE内容在XGF和宿主物种。这些发现表明需要进行详细的功能验证分析，以阐明单个TE插入的意义，并阐明TE-XGF热点的潜在机制。
The evolution of resistance is a major challenge for the sustainable control of pests and pathogens. Thus, a deeper understanding of the evolutionary and genomic mechanisms underpinning resistance evolution is required to safeguard health and food production. Several studies have implicated transposable elements (TEs) in xenobiotic-resistance evolution in insects. However, analyses are generally restricted to one insect species and/or one or a few xenobiotic gene families (XGFs). We examine evidence for TE accumulation at XGFs by performing a comparative genomic analysis across 20 aphid genomes, considering major subsets of XGFs involved in metabolic resistance to insecticides: cytochrome P450s, glutathione S-transferases, esterases, UDP-glucuronosyltransferases, and ABC transporters. We find that TEs are significantly enriched at XGFs compared with other genes. XGFs show similar levels of TE enrichment to those of housekeeping genes. But unlike housekeeping genes, XGFs are not constitutively expressed in germline cells, supporting the selective enrichment of TEs at XGFs rather than enrichment owing to chromatin availability. Hotspots of extreme TE enrichment occur around certain XGFs. We find, in aphids of agricultural importance, particular enrichment of TEs around cytochrome P450 genes with known functions in the detoxification of synthetic insecticides. Our results provide evidence supporting a general role for TEs as a source of genomic variation at host XGFs and highlight the existence of considerable variability in TE content across XGFs and host species. These findings show the need for detailed functional verification analyses to clarify the significance of individual TE insertions and elucidate underlying mechanisms at TE–XGF hotspots.
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