Bioengineered nisin A derivatives with enhanced activity against both Gram positive and Gram negative pathogens.
Bioengineered nisin A derivatives with enhanced activity against both Gram positive and Gram negative pathogens.
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DOI：
10.1371/journal.pone.0046884
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Ross RP
中科院分区：
文献类型：
--
作者：
Field D;Begley M;O'Connor PM;Daly KM;Hugenholtz F;Cotter PD;Hill C;Ross RP
乳酸链球菌素是一种细菌素，作为一种安全、天然的抗菌食品防腐剂，在50多个国家得到广泛应用。它是研究最广泛的细菌素，经历了几十年的生物工程，以期改善功能和理化性质。最近已经描述了对临床和食源性病原体具有增强活性的新型乳链菌肽变体的发现。我们筛选了一个随机的乳链菌肽A生产者库，并鉴定了一个在29位丝氨酸变为甘氨酸的变体（S29G），其对S.金黄色葡萄球菌SA113。使用位点饱和诱变方法，我们产生了另外三种衍生物（S29A、S29D和S29E），其对一系列革兰氏阳性耐药临床、兽医和食品病原体具有增强的活性。此外，当针对一系列革兰氏阴性食品相关病原体（包括大肠杆菌）进行评估时，许多乳链菌肽S29衍生物显示出上级乳链菌肽A的抗微生物活性。大肠杆菌、鼠伤寒沙门氏菌和坂崎克罗伊氏菌。这是第一次报道乳链菌肽衍生物，或实际上任何羊毛硫抗生素，具有增强的抗革兰氏阳性和革兰氏阴性细菌的抗微生物活性。
Nisin is a bacteriocin widely utilized in more than 50 countries as a safe and natural antibacterial food preservative. It is the most extensively studied bacteriocin, having undergone decades of bioengineering with a view to improving function and physicochemical properties. The discovery of novel nisin variants with enhanced activity against clinical and foodborne pathogens has recently been described. We screened a randomized bank of nisin A producers and identified a variant with a serine to glycine change at position 29 (S29G), with enhanced efficacy against S. aureus SA113. Using a site-saturation mutagenesis approach we generated three more derivatives (S29A, S29D and S29E) with enhanced activity against a range of Gram positive drug resistant clinical, veterinary and food pathogens. In addition, a number of the nisin S29 derivatives displayed superior antimicrobial activity to nisin A when assessed against a range of Gram negative food-associated pathogens, including E. coli, Salmonella enterica serovar Typhimurium and Cronobacter sakazakii. This is the first report of derivatives of nisin, or indeed any lantibiotic, with enhanced antimicrobial activity against both Gram positive and Gram negative bacteria.
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影响因子：
4.4
作者：
DODD, HM;HORN, N;GASSON, MJ
通讯作者：
GASSON, MJ
影响因子：
4.4
作者：
Hoffmann, A;Schneider, T;Sahl, HG
通讯作者：
Sahl, HG
DOI：
10.1111/j.1432-1033.1996.00267.x
发表时间：
1996-01-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Demel, RA;Peelen, T;Kuipers, OP
通讯作者：
Kuipers, OP
影响因子：
5.2
作者：
Brumfitt, W;Salton, MRJ;Hamilton-Miller, JMT
通讯作者：
Hamilton-Miller, JMT
影响因子：
5.2
作者：
Bonev, Boyan;Hooper, James;Parisot, Judicael
通讯作者：
Parisot, Judicael