Determination of immune factor levels in serum and local hematoma samples of osteoporotic fracture patients and clinical study of the effect of active vitamin D3 treatment on immune factor levels.
Determination of immune factor levels in serum and local hematoma samples of osteoporotic fracture patients and clinical study of the effect of active vitamin D3 treatment on immune factor levels.
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确定骨质疏松骨折患者的血清和局部血肿样品中的免疫因子水平以及活性维生素D3治疗对免疫因子水平的影响的临床研究。
DOI：
10.1186/s13018-023-03777-7
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发表时间：
2023-04-10
影响因子：
2.6
通讯作者：
Zhang, Mingwei
中科院分区：
文献类型：
--
作者：
Liu, Sijia;Li, Jianjun;Zhang, Mingwei
本研究的目的是观察骨折患者与非骨折患者全身和局部免疫因子白细胞介素（IL）-1β、IL-6和肿瘤坏死因子（TNF）-α的变化，探讨活性维生素D3治疗对骨折患者免疫功能和骨折预后的影响。术前检测IL-1β、IL-6和TNF-α mRNA表达水平。术后对照组采用常规骨折治疗加补钙，治疗组在常规骨折治疗的基础上加用钙剂加活性维生素D3。于术后第7天收集患者血清。骨折端血肿标本中IL-1β、IL-6和TNF-α 3种免疫因子的表达水平与血清中3种免疫因子的表达水平呈显著正相关（P < 0.05）。骨质疏松组血清IL-1β、IL-6和TNF-α的平均值显著高于非骨质疏松组（P < 0.05）。骨质疏松组平均血肿数明显高于非骨质疏松组（P < 0.05）。活性维生素D3治疗组的各项指标均显著低于对照组（P < 0.05）。活性维生素D3治疗组腕关节功能评分明显优于对照组（P < 0.05）。治疗组平均骨折愈合时间明显短于对照组（P < 0.05）。骨折端血肿标本中IL-1β、IL-6和TNF-α的相对表达量与血清中这些免疫因子的相应水平呈正相关。急性骨折患者血清和骨折端血肿标本中IL-1β、IL-6和TNF-α水平均高于非急性骨折患者。活性维生素D3治疗通过影响这些免疫因子的水平来促进骨折愈合。
The aim of this study was to investigate changes in systemic and local immune factors, namely, interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α, in patients with and without osteoporotic fractures and to explore the effects of active vitamin D3 treatment on immune function and fracture prognosis in patients with osteoporotic fractures. The mRNA expression levels of IL-1β, IL-6 and TNF-α were measured before the operation. After the operation, the patients in the control group were treated with conventional fracture treatment and calcium supplementation, and the patients in the treatment group were treated with calcium plus active vitamin D3 in addition to conventional fracture treatment. The serum of each patient was collected on the seventh day after the operation. The expression levels of the three immune factors (IL-1β, IL-6 and TNF-α) in the fracture end hematoma samples were significantly positively correlated with those in the serum samples (P < 0.05). The mean values of the serums of IL-1β, IL-6 and TNF-α in the osteoporosis group were significantly higher than those in the non-osteoporosis group (P < 0.05). The average number of hematomas in the osteoporosis group was significantly higher than that in the non-osteoporosis group (P < 0.05). The results for the active vitamin D3 treatment group were significantly lower than those for the control group (P < 0.05). The mean wrist function score of the active vitamin D3 treatment group was significantly better than that of the control group (P < 0.05). The average fracture healing time of the treatment group was significantly shorter than that of the control group (P < 0.05). The relative expression of IL-1β, IL-6, and TNF-α in the fracture end hematoma samples was positively correlated with the corresponding levels of these immune factors in the serum samples. The levels of IL-1β, IL-6 and TNF-α in the serum and fracture end hematoma samples of the osteoporotic fracture patients were higher than those of the non-osteoporotic fracture patients. Active vitamin D3 treatment promoted fracture healing by affecting the levels of these immune factors.
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影响因子：
9.3
作者：
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通讯作者：
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