Words of wisdom: re: characterization of the early proliferative response of the rodent bladder to subtotal cystectomy: a unique model of mammalian organ regeneration.
Words of wisdom: re: characterization of the early proliferative response of the rodent bladder to subtotal cystectomy: a unique model of mammalian organ regeneration.
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DOI：
10.1016/j.eururo.2012.11.018
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发表时间：
2013-02
期刊：
影响因子：
23.4
通讯作者：
Lue, Tom F.
中科院分区：
文献类型：
--
作者：
Lin, Ching-Shwun;Lue, Tom F.
在最近的一项研究[1]中，成年雌性大鼠接受次全膀胱切除术（STC;切除75%的膀胱），并在第1、3、5和7天，在收获膀胱进行组织学检查前2小时，腹腔内注射溴脱氧尿苷（BrdU）。在对照膀胱中，少于1%的细胞被BrdU标记，但在STC膀胱中，5 - 8%的细胞被标记。BrdU+细胞在STC膀胱中的分布，约65%在1天的上皮（EP）中，约80%在固有层（LP）和固有肌层（MP）中，然后在7天的三个隔室中相等。BrdU与细胞角蛋白，波形蛋白或平滑肌肌动蛋白（SMA）的共染色确定了上皮细胞，间质细胞和平滑肌细胞对STC诱导的增殖活性的贡献。然而，大多数BrdU+细胞缺乏这些细胞标志物，因此可能是祖细胞。STC还引起音刺猬（Shh）及其下游效应物Gli-1和BMP-4的染色增加，表明Shh通路参与膀胱再生。作者得出结论，STC膀胱是研究器官再生的分子和细胞机制的极好模型。
In a recent study [1], adult female rats were subjected to subtotal cystectomy (STC; removal of 75% of the bladder), and at 1, 3, 5 and 7 days, injected intraperitoneally with bromodeoxyuridine (BrdU) 2 hours before their bladders were harvested for histological examination. In control bladders, less than 1% of cells were labeled with BrdU, but in STC bladders between 5 and 8% were labeled. In regard to the distribution of BrdU+ cells in STC bladders,~ 65% were in the epithelium (EP) at 1-day,~ 80% in the lamina propria (LP) and muscularis propria (MP) at 3 and 5 days, and then equally in the three compartments at 7-day. Co-staining for BrdU with cytokeratin, vimentin or smooth muscle actin (SMA) identified the contribution of epithelial, insterstitial, and smooth muscle cells to the STC-induced proliferative activities. However, most BrdU+ cells were devoid of these cellular markers and thus are possibly progenitor cells. STC also caused an increase in staining for sonic hedgehog (Shh) and its downstream effectors Gli-1 and BMP-4, suggesting the involvement of the Shh pathway in bladder regeneration. The authors concluded that STC bladder is an excellent model for studying the molecular and cellular mechanisms of organ regeneration.
影响因子：
4.1
作者：
Burmeister, David;AbouShwareb, Tamer;Christ, George
通讯作者：
Christ, George
影响因子：
2.1
作者：
Zhang H;Lin G;Qiu X;Ning H;Banie L;Lue TF;Lin CS
通讯作者：
Lin CS