Deciphering neo-sex and B chromosome evolution by the draft genome of Drosophila albomicans.
Deciphering neo-sex and B chromosome evolution by the draft genome of Drosophila albomicans.
复制标题
通过白果蝇基因组草图破译新性别和 B 染色体进化
DOI：
10.1186/1471-2164-13-109
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发表时间：
2012-03-22
期刊：
影响因子：
4.4
通讯作者：
Bachtrog D
中科院分区：
文献类型：
--
作者：
Zhou Q;Zhu HM;Huang QF;Zhao L;Zhang GJ;Roy SW;Vicoso B;Xuan ZL;Ruan J;Zhang Y;Zhao RP;Ye C;Zhang XQ;Wang J;Wang W;Bachtrog D
背景
白腹果蝇是研究性染色体和B染色体进化的独特模式生物。一对约占整个基因组40%的常染色体在大约12万年前才与古老的X和Y染色体融合，从而创造了迄今报道的最年轻、基因最丰富的新性生殖系统。该物种还拥有最近衍生的B染色体，显示出非孟德尔遗传并显著影响生育能力。
方法
我们使用下一代测序技术对带有B染色体的雄性果蝇进行了124.5倍的基因组测序。为了表征neo-Y特异的变化和B染色体序列，我们还对来自同一品系但没有B染色体的近交系雌性果蝇进行了28.5倍的测序。
结果
我们组装了一个雌性基因组，并将53%的序列和85%的注释蛋白质放入特定的染色体，与12个果蝇基因组进行比较。尽管它的起源非常近，但非重组的neo-Y染色体显示出各种退化的迹象，包括与neo-X相比，非功能基因的显著丰富，以及相对于其他染色体的过度串联复制。我们还鉴定了一种B染色体连接的支架，它包含一个活跃的转录单位，并显示出与古代X染色体和新X染色体的亚着丝粒区域的序列相似性。
结论
我们的结果为性染色体进化和B染色体起源的早期阶段提供了新的见解，并表明这两个系统在白纹伊蚊的出生之间存在前所未有的联系。
Background
Drosophila albomicans is a unique model organism for studying both sex chromosome and B chromosome evolution. A pair of its autosomes comprising roughly 40% of the whole genome has fused to the ancient X and Y chromosomes only about 0.12 million years ago, thereby creating the youngest and most gene-rich neo-sex system reported to date. This species also possesses recently derived B chromosomes that show non-Mendelian inheritance and significantly influence fertility.
Methods
We sequenced male flies with B chromosomes at 124.5-fold genome coverage using next-generation sequencing. To characterize neo-Y specific changes and B chromosome sequences, we also sequenced inbred female flies derived from the same strain but without B's at 28.5-fold.
Results
We assembled a female genome and placed 53% of the sequence and 85% of the annotated proteins into specific chromosomes, by comparison with the 12 Drosophila genomes. Despite its very recent origin, the non-recombining neo-Y chromosome shows various signs of degeneration, including a significant enrichment of non-functional genes compared to the neo-X, and an excess of tandem duplications relative to other chromosomes. We also characterized a B-chromosome linked scaffold that contains an actively transcribed unit and shows sequence similarity to the subcentromeric regions of both the ancient X and the neo-X chromosome.
Conclusions
Our results provide novel insights into the very early stages of sex chromosome evolution and B chromosome origination, and suggest an unprecedented connection between the births of these two systems in D. albomicans.
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影响因子：
64.8
作者：
Clark, Andrew G.;Eisen, Michael B.;MacCallum, Iain
通讯作者：
MacCallum, Iain
DOI：
10.1098/rstb.2000.0717
发表时间：
2000-11-29
影响因子：
6.3
作者：
Charlesworth, B;Charlesworth, D
通讯作者：
Charlesworth, D
影响因子：
1.5
作者：
Bachtrog, Doris
通讯作者：
Bachtrog, Doris
果蝇米兰达（Drosophila Miranda）年轻Y染色体的基因组降解。
影响因子：
12.3
作者：
Bachtrog, Doris;Hom, Emily;Wong, Karen M.;Maside, Xulio;de Jong, Pieter
通讯作者：
de Jong, Pieter
影响因子：
4.5
作者：
Coleman JJ;Rounsley SD;Rodriguez-Carres M;Kuo A;Wasmann CC;Grimwood J;Schmutz J;Taga M;White GJ;Zhou S;Schwartz DC;Freitag M;Ma LJ;Danchin EG;Henrissat B;Coutinho PM;Nelson DR;Straney D;Napoli CA;Barker BM;Gribskov M;Rep M;Kroken S;Molnár I;Rensing C;Kennell JC;Zamora J;Farman ML;Selker EU;Salamov A;Shapiro H;Pangilinan J;Lindquist E;Lamers C;Grigoriev IV;Geiser DM;Covert SF;Temporini E;Vanetten HD
通讯作者：
Vanetten HD