Thalamocortical Projection Neuron and Interneuron Numbers in the Visual Thalamic Nuclei of the Adult C57BL/6 Mouse.
Thalamocortical Projection Neuron and Interneuron Numbers in the Visual Thalamic Nuclei of the Adult C57BL/6 Mouse.
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DOI：
10.3389/fnana.2018.00027
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发表时间：
2018
影响因子：
2.9
通讯作者：
Clascá F
中科院分区：
文献类型：
--
作者：
Evangelio M;García-Amado M;Clascá F
一个关键的参数约束预测，自下而上的电路模型的一个给定的大脑区域是涉及的神经元群的数量和位置。这些不仅包括身体位于区域内的神经元，还包括在遥远区域支配该区域的神经元。小鼠视觉皮层的主要皮层下输入来自膝状核背外侧（dLGN）和丘脑外侧后复合体。后者由三个不同的核组成：外侧后外侧核（LPL）、外侧后内侧吻侧核（LPMR）和外侧后内侧尾侧核（LPMC），每个核都表现出与不同视觉皮层区域的特定连接模式。在这里，我们确定了成年C57BL/6雄性小鼠LP复合体和dLGN中丘脑皮质投射神经元和中间神经元的数量。我们结合尼氏染色和组织化学和免疫标记方法来一致地描绘细胞核边界，并应用无偏的立体细胞计数方法。采用GABA免疫标记法鉴定丘脑中间神经元。C57BL/6 dLGN包含约21,200个神经元，而LP复合物包含约31,000个神经元。dLGN和LP是小鼠背丘脑中仅有的含有大量gaba免疫反应中间神经元的核。然而，这些中间神经元比之前估计的要少；它们占dLGN神经元的5.6%，只占LP神经元的1.9%。因此可以推断，dLGN含有~ 20,000个丘脑皮质投射神经元，LP复合物含有~ 30,400个丘脑皮质投射神经元（LPL为~ 12,000个，LPMR为15,200个，LPMC为4,200个）。目前的数据集与小鼠视觉丘脑皮质回路的约束模型相关，也与转基因小鼠品系之间或跨物种的定量比较相关。
A key parameter to constrain predictive, bottom-up circuit models of a given brain domain is the number and position of the neuronal populations involved. These include not only the neurons whose bodies reside within the domain, but also the neurons in distant regions that innervate the domain. The mouse visual cortex receives its main subcortical input from the dorsal lateral geniculate nucleus (dLGN) and the lateral posterior (LP) complex of the thalamus. The latter consists of three different nuclei: lateral posterior lateral (LPL), lateral posterior medial rostral (LPMR), and lateral posterior medial caudal (LPMC), each exhibiting specific patterns of connections with the various visual cortical areas. Here, we have determined the number of thalamocortical projection neurons and interneurons in the LP complex and dLGN of the adult C57BL/6 male mouse. We combined Nissl staining and histochemical and immunolabeling methods for consistently delineating nuclei borders, and applied unbiased stereological cell counting methods. Thalamic interneurons were identified using GABA immunolabeling. The C57BL/6 dLGN contains ∼21,200 neurons, while LP complex contains ∼31,000 total neurons. The dLGN and LP are the only nuclei of the mouse dorsal thalamus containing substantial numbers GABA-immunoreactive interneurons. These interneurons, however, are scarcer than previously estimated; they are 5.6% of dLGN neurons and just 1.9% of the LP neurons. It can be thus inferred that the dLGN contains ∼20,000 and the LP complex ∼30,400 thalamocortical projection neurons (∼12,000 in LPL, 15,200 in LPMR, and 4,200 in LPMC). The present dataset is relevant for constraining models of mouse visual thalamocortical circuits, as well as for quantitative comparisons between genetically modified mouse strains, or across species.
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影响因子：
2.5
作者：
Bermejo, PE;Jiménez, CE;Avendaño, C
通讯作者：
Avendaño, C
影响因子：
2
作者：
Gundersen, HJG;Jensen, EBV;Nielsen, J
通讯作者：
Nielsen, J
DOI：
10.1007/bf00325634
发表时间：
1971-01-01
期刊：
ZEITSCHRIFT FUR ZELLFORSCHUNG UND MIKROSKOPISCHE ANATOMIE
影响因子：
--
作者：
GENESERJENSEN, FA;BLACKSTAD, TW
通讯作者：
BLACKSTAD, TW
影响因子：
5.3
作者：
Bopp, Rita;Holler-Rickauer, Simone;Schuhknecht, Gregor F. P.
通讯作者：
Schuhknecht, Gregor F. P.
影响因子：
3.6
作者：
Hammer, Sarah;Carrillo, Gabriela L.;Fox, Michael A.
通讯作者：
Fox, Michael A.