Immune-related microRNAs are abundant in breast milk exosomes.
Immune-related microRNAs are abundant in breast milk exosomes.
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母乳外泌体中含有大量与免疫相关的 MicroRNA
DOI：
10.7150/ijbs.8.118
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发表时间：
2012
影响因子：
9.2
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Zhou Q;Li M;Wang X;Li Q;Wang T;Zhu Q;Zhou X;Wang X;Gao X;Li X
母乳是一种复杂的液体，富含影响婴儿免疫系统发育的免疫成分。外泌体是内吞起源的膜囊泡，其存在于各种体液中并且可以介导细胞间通讯。MicroRNA（miRNAs）是一组定义明确的非编码小RNA，包装在人类母乳中的外泌体内。在这里，我们使用深度测序技术从人类母乳外泌体中的452种miRNA前体（pre-miRNA）中鉴定出602种独特的miRNA。我们发现，在87种已充分表征的免疫相关前体miRNA中，有59种（67.82%）存在于母乳外泌体中并富集（P < 10-16，χ2检验）。此外，与外源性合成的miRNAs相比，这些内源性免疫相关miRNAs对相对苛刻的条件更具抵抗力。因此，很容易推测这些外泌体miRNA通过消化道从母乳转移到婴儿，并且它们在婴儿免疫系统的发育中发挥关键作用。
Breast milk is a complex liquid rich in immunological components that affect the development of the infant's immune system. Exosomes are membranous vesicles of endocytic origin that are found in various body fluids and that can mediate intercellular communication. MicroRNAs (miRNAs), a well-defined group of non-coding small RNAs, are packaged inside exosomes in human breast milk. Here, we identified 602 unique miRNAs originating from 452 miRNA precursors (pre-miRNAs) in human breast milk exosomes using deep sequencing technology. We found that, out of 87 well-characterized immune-related pre-miRNAs, 59 (67.82%) are presented and enriched in breast milk exosomes (P < 10-16, χ2 test). In addition, compared with exogenous synthetic miRNAs, these endogenous immune-related miRNAs are more resistant to relatively harsh conditions. It is, therefore, tempting to speculate that these exosomal miRNAs are transferred from the mother's milk to the infant via the digestive tract, and that they play a critical role in the development of the infant immune system.
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DOI：
10.1186/1758-907x-1-7
发表时间：
2010-03-01
期刊：
Silence
影响因子：
--
作者：
Kosaka N;Izumi H;Sekine K;Ochiya T
通讯作者：
Ochiya T
影响因子：
30.5
作者：
Stittrich, Anna-Barbara;Haftmann, Claudia;Mashreghi, Mir-Farzin
通讯作者：
Mashreghi, Mir-Farzin
影响因子：
3.7
作者：
Cheng H;Zhang L;Cogdell DE;Zheng H;Schetter AJ;Nykter M;Harris CC;Chen K;Hamilton SR;Zhang W
通讯作者：
Zhang W
影响因子：
3.7
作者：
Gilad S;Meiri E;Yogev Y;Benjamin S;Lebanony D;Yerushalmi N;Benjamin H;Kushnir M;Cholakh H;Melamed N;Bentwich Z;Hod M;Goren Y;Chajut A
通讯作者：
Chajut A
DOI：
10.1073/pnas.0804549105
发表时间：
2008-07-29
影响因子：
11.1
作者：
Mitchell, Patrick S.;Parkin, Rachael K.;Tewari, Muneesh
通讯作者：
Tewari, Muneesh