The effect of topography on differentiation fates of matrigel-coated mouse embryonic stem cells cultured on PLGA nanofibrous scaffolds.
The effect of topography on differentiation fates of matrigel-coated mouse embryonic stem cells cultured on PLGA nanofibrous scaffolds.
复制标题
地形对 PLGA 纳米纤维支架上培养的基质胶包被的小鼠胚胎干细胞分化命运的影响。
作者：
M. Massumi;M. Abasi;H. Babaloo;P. Terraf;Mojtaba Safi;Mahdi Saeed;J. Barzin;M. Zandi;M. Soleimani
由于胚胎干（ES）细胞的多能性，这些细胞是研究不同物理和化学因素对特化细胞分化/发育途径的影响的宝贵体外模型。我们寻求的影响，粗糙度和对齐，作为拓扑形态学特性的支架分化的绿色荧光蛋白表达ES细胞（GFP-ES）同时向三个胚层衍生物。此外，Matrigel作为天然细胞外基质与聚（乳酸-共-乙醇酸）（PLGA）纳米纤维支架组合对小鼠ES细胞分化的影响进行了研究。制备了不同高度、不同粗糙度分布和不同排列方式的PLGA纳米纤维支架。然后，通过基因表达谱分析将GFP-ES细胞接种在PLGA和PLGA/Matrigel支架上的不同细胞分化脂肪。研究结果表明，不同范围的粗糙度，高度和分布可以支持/促进支架上的特定细胞分化命运。用基质胶涂覆支架在中胚层衍生的细胞和生殖细胞从ES细胞分化中具有协同效应，而它抑制内胚层细胞谱系的衍生。结论是，拓扑形态学线索，如粗糙度和对齐，应考虑除了其他支架的性能，设计一个有效的静电纺丝支架的特定的组织工程。
Due to pluripotency of embryonic stem (ES) cells, these cells are an invaluable in vitro model that investigates the influence of different physical and chemical cues on differentiation/development pathway of specialized cells. We sought the effect of roughness and alignment, as topomorpholocial properties of scaffolds on differentiation of green fluorescent protein-expressing ES (GFP-ES) cells into three germ layers derivates simultaneously. Furthermore, the effect of Matrigel as a natural extracellular matrix in combination with poly(lactic-co-glycolic acid) (PLGA) nanofibrous scaffolds on differentiation of mouse ES cells has been investigated. The PLGA nanofibrous scaffolds with different height and distribution of roughness and alignments were fabricated. Then, the different cell differentiation fats of GFP-ES cells plated on PLGA and PLGA/Matrigel scaffolds were analyzed by gene expression profiling. The findings demonstrated that distinct ranges of roughness, height, and distribution can support/promote a specific cell differentiation fate on scaffolds. Coating of scaffolds with Matrigel has a synergistic effect in differentiation of mesoderm-derived cells and germ cells from ES cells, whereas it inhibits the derivation of endodermal cell lineages. It was concluded that the topomorpholocial cues such as roughness and alignment should be considered in addition to other scaffolds properties to design an efficient electrospun scaffold for specific tissue engineering.
登录
查看更多内容
影响因子：
14
作者：
Xie, Jingwei;Willerth, Stephanie M.;Li, Xiaoran;Macewan, Matthew R.;Rader, Allison;Sakiyama-Elbert, Shelly E.;Xia, Younan
通讯作者：
Xia, Younan
DOI：
10.1073/pnas.97.11.6126
发表时间：
2000-05-23
影响因子：
11.1
作者：
Liu, S;Qu, Y;McDonald, JW
通讯作者：
McDonald, JW
影响因子：
10.8
作者：
Patel, Shyam;Kurpinski, Kyle;Li, Song
通讯作者：
Li, Song
低温熔盐合成MoS2@CoS2异质结构以实现高效析氢反应
影响因子：
4.9
作者：
He, Song;Du, Hongfang;Huang, Wei
通讯作者：
Huang, Wei