Engineering the Surface Properties of a Zwitterionic Polymer Brush to Enable the Simple Fabrication of Inkjet-Printed Point-of-Care Immunoassays.
Engineering the Surface Properties of a Zwitterionic Polymer Brush to Enable the Simple Fabrication of Inkjet-Printed Point-of-Care Immunoassays.
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DOI：
10.1021/acs.langmuir.8b01597
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发表时间：
2019-02-05
期刊：
影响因子：
--
通讯作者：
Chilkoti A
中科院分区：
文献类型：
--
作者：
Fontes CM;Achar RK;Joh DY;Ozer I;Bhattacharjee S;Hucknall A;Chilkoti A
由于两性离子聚合物刷缺乏外来蛋白质吸附，从而保证了基于表面的免疫测定的低噪音，因此具有高分析灵敏度，我们探索了它们作为通过喷墨打印抗体来制造免疫测定的基质的用途。我们观察到，玻璃上的聚（磺基甜菜碱）甲基丙烯酸酯刷的亲水性太强，无法通过喷墨印刷实现抗体的非共价固定。为了克服这一限制，我们开发了一系列具有可调表面润湿性的混合两性离子-阳离子表面涂层，适用于抗体的喷墨打印，但蛋白质吸附也较低。我们表明，在微阵列格式中，捕获和检测抗体都被离散地打印为这些混合刷上的点，可以进行即时三明治免疫测定，其分析灵敏度和动态范围与在类 PEG 刷上制作的相同测定相似或更好。我们还表明，混合聚合物刷不结合人类血液中普遍存在的抗 PEG 抗体，这可能是在 PEG 类涂层上制造的免疫测定的问题。
Motivated by the lack of adventitious protein adsorption on zwitterionic polymer brushes that promise low noise and hence high analytical sensitivity for surface-based immunoassays, we explored their use as a substrate for immunoassay fabrication by inkjet printing of antibodies. We observed that a poly(sulfobetaine)methacrylate brush on glass is far too hydrophilic to enable non-covalent immobilization of antibodies by inkjet printing. To circumvent this limitation, we developed a series of hybrid zwitterionic-cationic surface coatings with tunable surface wettability that are suitable for inkjet printing of antibodies, but which also have low protein adsorption. We show that in a microarray format in which both capture and detection antibodies are discretely printed as spots on these hybrid brushes, a point-of-care sandwich immunoassay can be carried out with an analytical sensitivity and dynamic range that is similar to or better than the same assay fabricated on a PEG-like brush. We also show that the hybrid polymer brushes do not bind anti-PEG antibodies that are ubiquitous in human blood, which can be a problem with immunoassays fabricated on PEG-like coatings.
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DOI：
10.1002/jbm.820271105
发表时间：
1993-11-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
LLANOS, GR;SEFTON, MV
通讯作者：
SEFTON, MV
影响因子：
3.9
作者：
LENK, TJ;HORBETT, TA;CHITTUR, KK
通讯作者：
CHITTUR, KK
影响因子：
2.1
作者：
Hucknall, Angus;Simnick, Andrew J.;Ratner, Buddy D.
通讯作者：
Ratner, Buddy D.
DOI：
10.1163/156856202320253910
发表时间：
2002-01-01
影响因子：
3.6
作者：
Shen, MC;Martinson, L;Horbett, TA
通讯作者：
Horbett, TA
影响因子：
28.1
作者：
Qi, Yizhi;Simakova, Antonina;Chilkoti, Ashutosh
通讯作者：
Chilkoti, Ashutosh