Platelet-Rich Plasma: The Choice of Activation Method Affects the Release of Bioactive Molecules.
Platelet-Rich Plasma: The Choice of Activation Method Affects the Release of Bioactive Molecules.
复制标题
DOI：
10.1155/2016/6591717
复制
发表时间：
2016
影响因子：
--
通讯作者：
Filardo G
中科院分区：
文献类型：
--
作者：
Cavallo C;Roffi A;Grigolo B;Mariani E;Pratelli L;Merli G;Kon E;Marcacci M;Filardo G
富血小板血浆（PRP）是一种低成本的方法，可输送高浓度的自体生长因子（GFs）。血小板活化是可能影响生物活性分子可用性和组织愈合的关键步骤。通过添加10%的CaCl2、10%的自体凝血酶、10%的CaCl2 +凝血酶混合物和10%的i型胶原蛋白，激活10名自愿健康男性的PRP。血液衍生物孵育15和30分钟，1,2和24小时，评估样品的VEGF、TGF-β1、PDGF-AB、IL-1β和TNF-α的释放。用CaCl2、凝血酶和CaCl2/凝血酶激活的PRP在15分钟的评估中检测到形成血栓，而在i型胶原活化的样品中没有发现血栓形成。I型胶原蛋白产生整体较低的GF释放。凝血酶、CaCl2/凝血酶和I型胶原活化的PRPs显示PDGF和TGF-β 1的立即释放，随时间保持稳定，而VEGF在15分钟至24小时内呈增加趋势。CaCl2诱导GFs从15分钟开始逐渐释放，并增加至24小时。活化PRP的方法既影响其物理形态，也影响其GF量和释放动力学。
Platelet-Rich Plasma (PRP) is a low-cost procedure to deliver high concentrations of autologous growth factors (GFs). Platelet activation is a crucial step that might influence the availability of bioactive molecules and therefore tissue healing. Activation of PRP from ten voluntary healthy males was performed by adding 10% of CaCl2, 10% of autologous thrombin, 10% of a mixture of CaCl2 + thrombin, and 10% of collagen type I. Blood derivatives were incubated for 15 and 30 minutes and 1, 2, and 24 hours and samples were evaluated for the release of VEGF, TGF-β1, PDGF-AB, IL-1β, and TNF-α. PRP activated with CaCl2, thrombin, and CaCl2/thrombin formed clots detected from the 15-minute evaluation, whereas in collagen-type-I-activated samples no clot formation was noticed. Collagen type I produced an overall lower GF release. Thrombin, CaCl2/thrombin, and collagen type I activated PRPs showed an immediate release of PDGF and TGF-β 1 that remained stable over time, whereas VEGF showed an increasing trend from 15 minutes up to 24 hours. CaCl2 induced a progressive release of GFs from 15 minutes and increasing up to 24 hours. The method chosen to activate PRP influences both its physical form and the releasate in terms of GF amount and release kinetic.
登录
查看更多内容
ACL重建的PRP增强。
影响因子：
--
作者：
Andriolo L;Di Matteo B;Kon E;Filardo G;Venieri G;Marcacci M
通讯作者：
Marcacci M
影响因子：
3
作者：
Carreon, LY;Glassman, SD;Puno, RM
通讯作者：
Puno, RM
影响因子：
4.3
作者：
Bir, Shyamal Chandra;Esaki, Jiro;Sakata, Ryuzo
通讯作者：
Sakata, Ryuzo
影响因子：
1.9
作者：
Fufa, Duretti;Shealy, Blake;Murray, Martha M.
通讯作者：
Murray, Martha M.
影响因子：
1.9
作者：
Landesberg, R;Burke, A;Lu, HH
通讯作者：
Lu, HH