GA-responsive dwarfing gene Rht12 affects the developmental and agronomic traits in common bread wheat.
GA-responsive dwarfing gene Rht12 affects the developmental and agronomic traits in common bread wheat.
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DOI：
10.1371/journal.pone.0062285
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Hu YG
中科院分区：
文献类型：
--
作者：
Chen L;Phillips AL;Condon AG;Parry MA;Hu YG
存在用替代矮秆基因（如赤霉素反应基因Rht 12）取代降低高度（Rht）基因Rht-B1 b和Rht-D1 b的机会，用于面包小麦改良。然而，对Rht 12的作用和作用方式缺乏全面的了解。本研究通过分析Rht 12对小麦幼苗活力、幼苗根系、茎叶形态、穗部发育和碳水化合物同化与分配的影响来表征Rht 12的效应。这是在宁春45与Karcagi（12）杂交的F2：3系的四个基因型中进行的秋播和春播两个试验。Rht 12显著降低了茎长（43%~ 48%）和剑叶长（25%~ 30%），增加了节间壁厚度和叶宽。虽然Rht 12使最终株高缩短40%，但对幼苗活力，特别是胚芽鞘长度和苗期根系性状没有不利影响。在秋播试验中，Rht 12延长了穗发育期，提高了开花期穗干重比例，显著提高了小花育性（14%）。然而，Rht 12延迟开花日期约5天，即使是显性Vrn-B1等位基因也不能补偿这种负面影响。此外，Rht 12株系花后支持穗发育的能力下降，籽粒尺寸减小。最后，在秋播试验中，矮秆系和高秆系之间的籽粒产量相似。因此，Rht 12可以显著降低株高而不改变种子活力，并显着提高小穗育性在良好的秋播环境。Rht 12在育种计划中的成功利用将需要仔细选择，因为它可能会延迟穗的出现。尽管如此，通过使用Rht 12对小麦进行改良的潜力仍然存在。
Opportunities exist for replacing reduced height (Rht) genes Rht-B1b and Rht-D1b with alternative dwarfing genes, such as the gibberellin-responsive gene Rht12, for bread wheat improvement. However, a comprehensive understanding of the effects and mode of action of Rht12 is lacking. In the present study, the effects of Rht12 were characterized by analyzing its effects on seeding vigour, seedling roots, leaf and stem morphology, spike development and carbohydrate assimilation and distribution. This was carried out in the four genotypes of F2:3 lines derived from a cross between Ningchun45 and Karcagi (12) in two experiments of autumn sowing and spring sowing. Rht12 significantly decreased stem length (43%∼48% for peduncle) and leaf length (25%∼30% for flag leaf) while the thickness of the internode walls and width of the leaves were increased. Though the final plant stature was shortened (40%) by Rht12, the seedling vigour, especially coleoptile length and root traits at the seedling stage, were not affected adversely. Rht12 elongated the duration of the spike development phase, improved the proportion of spike dry weight at anthesis and significantly increased floret fertility (14%) in the autumn sowing experiment. However, Rht12 delayed anthesis date by around 5 days and even the dominant Vrn-B1 allele could not compensate this negative effect. Additionally, grain size was reduced with the ability to support spike development after anthesis decreased in Rht12 lines. Finally, grain yield was similar between the dwarf and tall lines in the autumn sowing experiment. Thus, Rht12 could substantially reduce plant height without altering seeding vigour and significantly increase spikelet fertility in the favourable autumn sowing environment. The successful utilization of Rht12 in breeding programs will require careful selection since it might delay ear emergence. Nonetheless, the potential exists for wheat improvement by using Rht12.
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DOI：
10.1071/pp9800169
发表时间：
1980-01-01
期刊：
AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY
影响因子：
--
作者：
FISCHER, RA;STOCKMAN, YM
通讯作者：
STOCKMAN, YM
影响因子：
2.1
作者：
HAUN, JR
通讯作者：
HAUN, JR
影响因子：
2
作者：
Flintham, JE;Borner, A;Gale, MD
通讯作者：
Gale, MD
影响因子：
1.9
作者：
González, FG;Slafer, GA;Miralles, DJ
通讯作者：
Miralles, DJ
影响因子：
2.3
作者：
ALLAN, RE
通讯作者：
ALLAN, RE