Genome-wide analysis of the WRKY gene family in the cucumber genome and transcriptome-wide identification of WRKY transcription factors that respond to biotic and abiotic stresses.
Genome-wide analysis of the WRKY gene family in the cucumber genome and transcriptome-wide identification of WRKY transcription factors that respond to biotic and abiotic stresses.
复制标题
对黄瓜基因组中 WRKY 基因家族进行全基因组分析，并对响应生物和非生物胁迫的 WRKY 转录因子进行全转录组鉴定。
DOI：
10.1186/s12870-020-02625-8
复制
发表时间：
2020-09-25
影响因子：
5.3
通讯作者：
Ren Z
中科院分区：
文献类型：
--
作者：
Chen C;Chen X;Han J;Lu W;Ren Z
背景
黄瓜(Cucumis sativus L.)是一种重要的经济作物品种。然而，它对各种非生物和生物胁迫都很敏感。WRKY转录因子在植物生长发育中发挥重要作用，特别是在植物对生物和非生物胁迫的响应中。然而，关于WRKY基因在不同胁迫下在黄瓜中的表达模式还知之甚少。
结果
在本研究中，对黄瓜基因组(v3.0)的新组装的分析允许鉴定61个黄瓜WRKY基因。利用近缘种对黄瓜WRKY基因进行了系统发育和同源性分析。61个CsWRKY被分为三大类，其中基因结构和基序组成是保守的。WRKY基因的组织表达谱表明，24个WRKY基因在所有样本中都表现为组成性表达(所有样本中都有FPKM 1)，部分WRKY基因表现出器官特异性表达，这表明这些WRKY基因可能对黄瓜的植物生长和器官发育具有重要作用。重要的是，对CsWRKY基因表达谱的分析表明，5个CsWRKY基因对盐胁迫和高温胁迫都有强烈的反应，12个基因对霜霉病和白粉病的侵染有反应，3个CsWRKY基因同时对所分析的所有处理做出反应。一些CsWRKY基因在非生物或生物胁迫处理后的不同时间被诱导/抑制，这表明黄瓜WRKY基因在不同的胁迫响应中可能发挥不同的作用，其表达模式随胁迫的不同而不同。
结论
本研究在黄瓜中鉴定了61个WRKY基因，并对其分类、进化和表达模式进行了深入的研究。还研究了黄瓜对不同非生物和生物胁迫的响应。我们的结果为更好地理解CsWRKY基因在提高黄瓜非生物和生物抗逆性方面的功能提供了更好的理解。
Background
Cucumber (Cucumis sativus L.) is an economically important vegetable crop species. However, it is susceptible to various abiotic and biotic stresses. WRKY transcription factors play important roles in plant growth and development, particularly in the plant response to biotic and abiotic stresses. However, little is known about the expression pattern of WRKY genes under different stresses in cucumber.
Results
In the present study, an analysis of the new assembly of the cucumber genome (v3.0) allowed the identification of 61 cucumber WRKY genes. Phylogenetic and synteny analyses were performed using related species to investigate the evolution of the cucumber WRKY genes. The 61 CsWRKYs were classified into three main groups, within which the gene structure and motif compositions were conserved. Tissue expression profiles of the WRKY genes demonstrated that 24 CsWRKY genes showed constitutive expression (FPKM 1 in all samples), and some WRKY genes showed organ-specific expression, suggesting that these WRKYs might be important for plant growth and organ development in cucumber. Importantly, analysis of the CsWRKY gene expression patterns revealed that five CsWRKY genes strongly responded to both salt and heat stresses, 12 genes were observed to be expressed in response to infection from downy mildew and powdery mildew, and three CsWRKY genes simultaneously responded to all treatments analysed. Some CsWRKY genes were observed to be induced/repressed at different times after abiotic or biotic stress treatment, demonstrating that cucumber WRKY genes might play different roles during different stress responses and that their expression patterns vary in response to stresses.
Conclusions
Sixty-one WRKY genes were identified in cucumber, and insight into their classification, evolution, and expression patterns was gained in this study. Responses to different abiotic and biotic stresses in cucumber were also investigated. Our results provide a better understanding of the function of CsWRKY genes in improving abiotic and biotic stress resistance in cucumber.
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影响因子：
56.9
作者：
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通讯作者：
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影响因子：
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作者：
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