OsSERK1 regulates rice development but not immunity to Xanthomonas oryzae pv. oryzae or Magnaporthe oryzae.
OsSERK1 regulates rice development but not immunity to Xanthomonas oryzae pv. oryzae or Magnaporthe oryzae.
复制标题
DOI：
10.1111/jipb.12290
复制
发表时间：
2014-12
影响因子：
11.4
通讯作者：
Ronald PC
中科院分区：
文献类型：
--
作者：
Zuo S;Zhou X;Chen M;Zhang S;Schwessinger B;Ruan D;Yuan C;Wang J;Chen X;Ronald PC
体细胞胚胎发生受体激酶（SERK）蛋白在植物发育和免疫调节中起着重要作用。水稻基因组包含两个SERK基因，OsSerk1和OsSerk2。我们以前证明OsSerk2是水稻Xa21介导的抗黄单胞菌黄单胞菌所必需的。Xoo）和正常发育。在这里，我们报告的OsSerk1的分子特征。OsSerk1的过表达导致半矮化表型，而OsSerk1的沉默导致叶片接合角度减小。OsSerk 1不是水稻抗Xoo和Magnaporthe magnesium（M.）。OsSerk2沉默株系中OsSerk1的过表达补充了与油菜素类固醇（BR）信号传导缺陷相关的表型，但不是由Xa21介导的抗病表型。在酵母中，OsSERK1与自身相互作用形成同源二聚体，并且还分别与OsSERK2和BRI1的激酶结构域相互作用。OsSERK1是一种具有自磷酸化功能的蛋白激酶。因此，OsSERK2在水稻发育和免疫过程中起重要作用，而OsSERK1在水稻发育过程中起重要作用，但在水稻对Xoo和M的免疫过程中不起作用。米。
Somatic embryogenesis receptor kinase (SERK) proteins play pivotal roles in regulation of plant development and immunity. The rice genome contains two SERK genes, OsSerk1 and OsSerk2. We previously demonstrated that OsSerk2 is required for rice Xa21-mediated resistance to Xanthomonas oryzae pv. oryzae (Xoo) and for normal development. Here we report the molecular characterization of OsSerk1. Overexpression of OsSerk1 results in a semi-dwarf phenotype whereas silencing of OsSerk1 results in a reduced angle of the lamina joint. OsSerk1 is not required for rice resistance to Xoo or Magnaporthe oryzae (M. oryzae). Overexpression of OsSerk1 in OsSerk2-silenced lines complements phenotypes associated with brassinosteroid (BR) signaling defects, but not the disease resistance phenotype mediated by Xa21. In yeast, OsSERK1 interacts with itself forming homodimers, and also interacts with the kinase domains of OsSERK2 and BRI1, respectively. OsSERK1 is a functional protein kinase capable of auto-phosphorylation in vitro. We conclude that, whereas OsSERK2 regulates both rice development and immunity, OsSERK1 functions in rice development but not immunity to Xoo and M. oryzae.
登录
查看更多内容
影响因子：
64.8
作者：
Chinchilla, Delphine;Zipfel, Cyril;Boller, Thomas
通讯作者：
Boller, Thomas
影响因子：
64.5
作者：
Li, J;Wen, JQ;Walker, JC
通讯作者：
Walker, JC
影响因子：
11.6
作者：
Roux, Milena;Schwessinger, Benjamin;Zipfel, Cyril
通讯作者：
Zipfel, Cyril
体细胞胚胎发生受体激酶在油菜素类固醇信号传导中不可或缺作用的遗传证据
影响因子：
4.5
作者：
Gou X;Yin H;He K;Du J;Yi J;Xu S;Lin H;Clouse SD;Li J
通讯作者：
Li J
影响因子：
4.3
作者：
Hu, H;Xiong, L;Yang, Y
通讯作者：
Yang, Y