Controlling and co-ordinating chitinase secretion in a Serratia marcescens population
Controlling and co-ordinating chitinase secretion in a Serratia marcescens population
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控制和协调粘质沙雷氏菌群体中的几丁质酶分泌
DOI：
10.1101/652685
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
De Assis Alcoforado Costa M
中科院分区：
文献类型：
--
作者：
De Assis Alcoforado Costa M
粘质沙雷氏菌是一种γ-变形杆菌属，也是一种机会性动物和昆虫病原体。该细菌表现出复杂的细胞外蛋白质“分泌组”，包括许多酶、毒素和效应分子。分泌组的一个组分是“几丁质分解机制”，其是一组至少四种几丁质酶，其允许使用不溶性细胞外几丁质作为唯一的碳源和氮源。几丁质酶跨外膜的分泌受编码holin/内肽酶对的chiWXYZ操纵子控制。chiWXYZ操纵子的表达与几丁质酶基因协调，并且也是双峰的，因为通常只有1%的群体表达几丁质分解机制。在这项工作中，ChiR蛋白在几丁质酶生产中的作用进行了探索。使用活细胞成像和流式细胞术，ChiR被证明可以控制chiWXYZ与bothchiA和chiC的协调调节。此外，chiRalone的过表达能够将表达几丁质酶基因的细胞群体的比例增加至> 60%。此外，定量标记的蛋白质组学分析细胞overexpressingChiR建立，ChiR调节整个几丁质分解机制。蛋白质组学实验还揭示了几丁质分解机制的调节与参与氮化合物（如硝酸盐和亚硝酸盐）代谢的蛋白质的产生之间的惊人联系。该研究首次证明ChiR在控制粘质沙雷氏菌双峰基因表达方面发挥着关键作用，并提供了新的证据证明甲壳素分解与氮代谢之间存在明确的联系。重要信息机会致病菌粘质沙雷氏菌通过chiWXYZoperon的作用分泌甲壳素酶，该操纵子编码一个洞蛋白/内肽酶对。chiWXYZ的表达通常是双峰的，只有1%的人转录这些基因。在这项工作中，研究表明ChiR的过表达会诱导大多数细胞群体中紧密协调的孔蛋白/内肽酶和几丁质酶基因表达，从而确定ChiR是生物模式基因表达中的关键参与者。这项工作还首次揭示了ChiR诱导的合作途径，包括参与氨，亚硝酸盐和硝酸盐代谢的酶。这项工作扩展了基本细菌生理学的知识，并可能在生物医学和生物技术研究领域的应用。
Serratia marcescensis a γ-Proteobacterium and an opportunistic animal and insect pathogen. The bacterium exhibits a complex extracellular protein ‘secretome’ comprising numerous enzymes, toxins and effector molecules. One component of the secretome is the ‘chitinolytic machinery’, which is a set of at least four chitinases that allow the use of insoluble extracellular chitin as sole carbon and nitrogen source. Secretion of the chitinases across the outer membrane is governed by thechiWXYZoperon encoding a holin/endopeptidase pair. Expression of thechiWXYZoperon is co-ordinated with the chitinase genes and is also bimodal, since normally only 1% of the population expresses the chitinolytic machinery. In this work, the role of the ChiR protein in chitinase production has been explored. Using live cell imaging and flow cytometry, ChiR was shown to govern the co-ordinated regulation ofchiWXYZwith bothchiAandchiC. Moreover, overexpression ofchiRalone was able to increase the proportion of the cell population expressing chitinase genes to >60%. In addition, quantitative label-free proteomic analysis of cells overexpressingchiRestablished that ChiR regulates the entire chitinolytic machinery. The proteomic experiments also revealed a surprising link between the regulation of the chitinolytic machinery and the production of proteins involved in the metabolism of nitrogen compounds such as nitrate and nitrite. The research demonstrates for the first time that ChiR plays a critical role in controlling bimodal gene expression inS. marcescens, and provides new evidence of a clear link between chitin breakdown and nitrogen metabolism.IMPORTANCEThe opportunistic pathogenSerratia marcescenssecretes chitinases through the action of thechiWXYZoperon, which encodes a holin/endopeptidase pair. Expression ofchiWXYZis normally bimodal, with only 1% of the population transcribing these genes. In this work, it is demonstrated that overexpression ofchiRinduces exquisitely co-ordinated holin/endopeptidase and chitinase gene expression in the majority of the population of cells, establishing that ChiR is a key player in biomodal gene expression. This work also reveals for the first time that co-operating pathways are induced by ChiR, including enzymes involved in ammonia, nitrite and nitrate metabolism. This work expands knowledge of basic bacterial physiology and could have applications in the biomedical and biotechnological research fields.
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通过磷酸转移酶系统摄取 N,N-二乙酰壳二糖 [(GlcNAc)2] 对于粘质沙雷氏菌 2170 生产几丁质酶至关重要
影响因子：
3.2
作者：
T. Uchiyama;R. Kaneko;J. Yamaguchi;Akane Inoue;Takahiro Yanagida;N. Nikaidou;M. Regué;Takeshi Watanabe
通讯作者：
Takeshi Watanabe
粘质沙雷氏菌的昆虫致病特性：噬菌体抗性突变体，对天蚕免疫的抵抗力降低，对果蝇的毒力降低。
DOI：
10.1099/00221287-120-1-173
发表时间：
1980
期刊：
Journal of general microbiology
影响因子：
--
作者：
C. Flyg;K. Kenne;H. G. Boman
通讯作者：
H. G. Boman
DOI：
10.1083/jcb.201404127
发表时间：
2014-12-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hamilton JJ;Marlow VL;Owen RA;Costa Mde A;Guo M;Buchanan G;Chandra G;Trost M;Coulthurst SJ;Palmer T;Stanley-Wall NR;Sargent F
通讯作者：
Sargent F
LysR 型转录调节因子 ChiR 对于粘质沙雷氏菌 2170 中所有几丁质酶和几丁质结合蛋白 CBP21 的产生至关重要
DOI：
--
发表时间：
2001
期刊：
Bioscience, biotechnology and biochemistry
影响因子：
--
作者：
Kazushi Suzuki;Takuma Uchiyama;Megumi Suzuki;N. Nikaidou;M. Regué;Takeshi Watanabe
通讯作者：
Takeshi Watanabe
弯曲细菌：变形菌中细胞形状机制的比较。
影响因子：
10.5
作者：
Taylor,JenniferA;Sichel,SophieR;Salama,NinaR
通讯作者：
Salama,NinaR