A thermodynamic overview of naturally occurring intramolecular DNA quadruplexes.
A thermodynamic overview of naturally occurring intramolecular DNA quadruplexes.
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DOI：
10.1093/nar/gkn543
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发表时间：
2008-10
影响因子：
14.9
通讯作者：
Maiti, Souvik
中科院分区：
文献类型：
--
作者：
Kumar, Niti;Maiti, Souvik
环长度及其组成对于四链体的结构和功能多样性是重要的。迄今为止，对环的研究主要关注与天然存在的具有不同环长度和组成的四链体序列相比的模型序列。在此，我们已经确定了36个四链体形成序列的启动子区的各种原癌基因，使用CD，UV和天然凝胶电泳。我们研究了折叠拓扑结构，并确定了不同的总环长（5-18个碱基）和组成的四链体的热力学概况。我们发现，天然存在的四链体具有可变的热力学稳定性（ΔG37），范围为−1.7至−15.6 kcal/mol。总的来说，我们的研究结果表明，环的长度和它的组成影响四链体结构和热力学，从而使它难以绘制环的长度和热力学稳定性之间的广义相关性。此外，我们比较了四链体和它们各自的双链体的热力学稳定性，以了解四链体-双链体竞争。我们的研究结果引发了一个讨论，生物功能是否与四链体具有较低的热力学稳定性，经历容易的形成和破坏，或四链体具有较高的热力学稳定性。
Loop length and its composition are important for the structural and functional versatility of quadruplexes. To date studies on the loops have mainly concerned model sequences compared with naturally occurring quadruplex sequences which have diverse loop lengths and compositions. Herein, we have characterized 36 quadruplex-forming sequences from the promoter regions of various proto-oncogenes using CD, UV and native gel electrophoresis. We examined folding topologies and determined the thermodynamic profile for quadruplexes varying in total loop length (5–18 bases) and composition. We found that naturally occurring quadruplexes have variable thermodynamic stabilities (ΔG37) ranging from −1.7 to −15.6 kcal/mol. Overall, our results suggest that both loop length and its composition affect quadruplex structure and thermodynamics, thus making it difficult to draw generalized correlations between loop length and thermodynamic stability. Additionally, we compared the thermodynamic stability of quadruplexes and their respective duplexes to understand quadruplex–duplex competition. Our findings invoke a discussion on whether biological function is associated with quadruplexes with lower thermodynamic stability which undergo facile formation and disruption, or by quadruplexes with high thermodynamic stability.
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影响因子：
14.9
作者：
Kumar N;Maiti S
通讯作者：
Maiti S
影响因子：
11.4
作者：
Cortés, A;Huertas, D;Azorín, F
通讯作者：
Azorín, F
DOI：
10.1016/j.bbrc.2004.05.052
发表时间：
2004-07-02
影响因子：
3.1
作者：
Kumar, N;Maiti, S
通讯作者：
Maiti, S
影响因子：
3.3
作者：
Kumar, Niti;Maiti, Souvik
通讯作者：
Maiti, Souvik
影响因子：
3.9
作者：
Guedin, Aurore;De Cian, Anne;Mergny, Jean-Louis
通讯作者：
Mergny, Jean-Louis