CUTI-1: A novel tetraspan protein involved in C. elegans CUTicle formation and epithelial integrity.
CUTI-1: A novel tetraspan protein involved in C. elegans CUTicle formation and epithelial integrity.
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DOI：
10.1371/journal.pone.0005117
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Behm CA
中科院分区：
文献类型：
--
作者：
Fritz JA;Behm CA
线虫角质层是坚韧的细胞外基质，主要由交联胶原蛋白和非胶原角质素组成。它是线虫运动和保护免受外部环境影响所必需的。人们对角质层形成的复杂过程如何适应线虫角质层的特殊要求知之甚少，线虫角质层在结构和组成上都不同于其他生物体。线虫基因 cuti-1（CUTicle 和上皮完整性）编码线虫特异性蛋白。我们已经证明 CUTI-1 在上皮细胞和接缝细胞中表达。在这些组织中，cuti-1 mRNA 的表达在整个发育过程中循环，与蜕皮周期一致，这一过程涉及新角质层的合成。此外，通过RNA干扰（RNAi）敲低cuti-1会导致线虫表现出与角质层功能障碍和上皮完整性缺陷相关的胚胎后表型。这是有关线虫特异性蛋白质参与细胞外基质形成的最早报道之一。它进一步深入了解了如何进化出调节角质层形成的新方法，角质层是线虫的主要保护屏障和骨骼组成部分。
The nematode cuticle is a tough extracellular matrix composed primarily of cross-linked collagens and non-collagenous cuticulins. It is required for nematode motility and protection from the external environment. Little is known about how the complex process of cuticle formation has been adapted to the specialized requirements of the nematode cuticle, which is structurally and compositionally unique from other organisms. The C. elegans gene cuti-1 (CUTicle and epithelial Integrity) encodes a nematode-specific protein. We have shown that CUTI-1 is expressed in the epithelia and in seam cells. Within these tissues the expression of cuti-1 mRNA cycles throughout development in line with the molting cycle, a process that involves synthesis of a new cuticle. In addition, knockdown of cuti-1 by RNA interference (RNAi) results in worms that display post-embryonic phenotypes related to cuticle dysfunction and defects in epithelial integrity. This is one of the first reports of a nematode-specific protein involved in extracellular matrix formation. It provides further insight into how novel ways have evolved to regulate the formation of the cuticle, which is the primary protective barrier and skeletal component of nematodes.
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DOI：
10.1083/jcb.146.3.659
发表时间：
1999-08-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hresko MC;Schriefer LA;Shrimankar P;Waterston RH
通讯作者：
Waterston RH
影响因子：
5.3
作者：
BIRD, DM;RIDDLE, DL
通讯作者：
RIDDLE, DL
DOI：
10.1073/pnas.96.11.6241
发表时间：
1999-05-25
影响因子：
11.1
作者：
Cheong, KH;Zacchetti, D;Simons, K
通讯作者：
Simons, K
影响因子：
1.5
作者：
Johnstone, IL;Shafi, Y;Barry, JD
通讯作者：
Barry, JD
影响因子：
9.2
作者：
Michaux, G;Gansmuller, A;Labouesse, M
通讯作者：
Labouesse, M