3D-printed microfluidic device for the detection of pathogenic bacteria using size-based separation in helical channel with trapezoid cross-section.
3D-printed microfluidic device for the detection of pathogenic bacteria using size-based separation in helical channel with trapezoid cross-section.
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DOI：
10.1038/srep07717
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发表时间：
2015-01-12
影响因子：
4.6
通讯作者：
Jeon S
中科院分区：
文献类型：
--
作者：
Lee W;Kwon D;Choi W;Jung GY;Jeon S
已经开发了一种使用磁性纳米颗粒簇（MNCs）和3D打印螺旋微通道检测病原菌的简便方法。抗体功能化的MNCs用于捕获E.大肠杆菌（EC）细菌的牛奶中，和游离的MNCs和MNC-EC复合物分离使用永磁体。将游离的MNCs和MNC-EC复合物分散在缓冲溶液中，然后将该溶液注射到具有或不具有鞘流的螺旋微通道装置中。MNC-EC复合物通过Dean拖曳力和升力与游离MNCs分离，并且在鞘流的存在下促进分离。E.用分光光度计测定大肠杆菌，缓冲液中检出限为10 cfu/mL，牛奶中检出限为100 cfu/mL。
A facile method has been developed to detect pathogenic bacteria using magnetic nanoparticle clusters (MNCs) and a 3D-printed helical microchannel. Antibody-functionalized MNCs were used to capture E. coli (EC) bacteria in milk, and the free MNCs and MNC-EC complexes were separated from the milk using a permanent magnet. The free MNCs and MNC-EC complexes were dispersed in a buffer solution, then the solution was injected into a helical microchannel device with or without a sheath flow. The MNC-EC complexes were separated from the free MNCs via the Dean drag force and lift force, and the separation was facilitated in the presence of a sheath flow. The concentration of the E. coli bacteria was determined using a light absorption spectrometer, and the limit of detection was found to be 10 cfu/mL in buffer solution and 100 cfu/mL in milk.
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影响因子：
4.2
作者：
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通讯作者：
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影响因子：
6.1
作者：
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影响因子：
4.3
作者：
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影响因子：
3.7
作者：
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通讯作者：
Asmolov, ES
DOI：
10.1073/pnas.0704958104
发表时间：
2007-11-27
影响因子：
11.1
作者：
Di Carlo, Dino;Irimia, Daniel;Toner, Mehmet
通讯作者：
Toner, Mehmet