Claudin-4 localization in epithelial ovarian cancer.
Claudin-4 localization in epithelial ovarian cancer.
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DOI：
10.1016/j.heliyon.2022.e10862
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发表时间：
2022-10
期刊：
影响因子：
4
通讯作者：
Bitler, Benjamin G.
中科院分区：
文献类型：
--
作者：
Neville, Margaret C.;Webb, Patricia G.;Baumgartner, Heidi K.;Bitler, Benjamin G.
关键词：
Claudin-4是一种具有经典claudin结构的蛋白质，最常在细胞-细胞连接中发现，在上皮癌中经常过表达，其定位尚未研究。在这项研究中，我们的目的是找出这种膜蛋白在卵巢肿瘤模型OVCAR 3细胞中的定位，OVCAR 3细胞表达高水平的蛋白质。免疫组织化学研究表明，claudin-4染色在核周区域，在大多数质膜和细胞质点。天然密蛋白-4与磷酸化密蛋白-4不重叠，磷酸化密蛋白-4部分位于局灶性粘连中。使用claudin-4 BioID技术，我们证实了大量的claudin-4定位于高尔基体区室，包括在细胞中的分散的高尔基体中，其中claudin-4被部分敲低和分裂细胞中。Claudin-4似乎存在于几种类型的细胞-细胞连接附近，但没有证据表明它在这些肿瘤细胞中形成紧密连接。紧密连接蛋白4、高尔基体标记物GM 130和质膜受体Notch 2均存在于分裂细胞中分散的高尔基体中。这种对claudin-4细胞结构的定义应该为更好地理解claudin-4在肿瘤细胞中的功能及其分子相互作用提供一个框架。紧密连接蛋白-4;卵巢癌;高尔基体;内质网;核内体;细胞间接触;质膜蛋白质类;细胞结构。
Claudin-4, a protein with the structure of classic claudins most often found in cell-cell junctions, is frequently overexpressed in epithelial cancers where its localization has not been studied. In this study we aimed to find out where this membrane protein is localized in an ovarian tumor model, OVCAR3 cells, that express high levels of the protein. Immunohistochemical studies showed claudin-4 staining in a perinuclear region, at most plasma membranes and in cytoplasmic puncta. Native claudin-4 did not overlap with phosphorylated claudin-4, which was partially located in focal adhesions. Using claudin-4 BioID technology we confirmed that large amounts of claudin-4 are localized to the Golgi compartment, including in dispersed Golgi in cells where claudin-4 is partially knocked down and in dividing cells. Claudin-4 appears to be present in the vicinity of several types of cell-cell junctions, but there is no evidence that it forms tight junctions in these tumor cells. Both claudin-4, the Golgi marker GM130, and the plasma membrane receptor Notch2 were found in dispersed Golgi in dividing cells. This definition of the cellular architecture of claudin-4 should provide a framework for better understanding of the function of claudin-4 in tumor cells and its molecular interactions. Claudin-4; Ovarian cancer; Golgi; Endoplasmic reticulum; Endosome; Cell-cell contacts; Plasma membrane proteins; Cell architecture.
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DOI：
10.1083/jcb.145.1.83
发表时间：
1999-04-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Infante C;Ramos-Morales F;Fedriani C;Bornens M;Rios RM
通讯作者：
Rios RM
三个紧密连接相关的Maguks ZO-1，ZO-2和ZO-3与Claudins的Cooh Termini直接结合。
DOI：
10.1083/jcb.147.6.1351
发表时间：
1999-12-13
期刊：
The Journal of cell biology
影响因子：
--
作者：
Itoh M;Furuse M;Morita K;Kubota K;Saitou M;Tsukita S
通讯作者：
Tsukita S
影响因子：
3.8
作者：
Hicks DA;Galimanis CE;Webb PG;Spillman MA;Behbakht K;Neville MC;Baumgartner HK
通讯作者：
Baumgartner HK
影响因子：
21.3
作者：
Anitei, Mihaela;Stange, Christoph;Parshina, Irina;Baust, Thorsten;Schenck, Annette;Raposo, Graca;Kirchhausen, Tomas;Hoflack, Bernard
通讯作者：
Hoflack, Bernard
影响因子：
--
作者：
Aktary Z;Alaee M;Pasdar M
通讯作者：
Pasdar M