Development of an Enzyme-Linked Immunosorbent Assay to Determine the Expression Dynamics of Ebola Virus Soluble Glycoprotein during Infection.
Development of an Enzyme-Linked Immunosorbent Assay to Determine the Expression Dynamics of Ebola Virus Soluble Glycoprotein during Infection.
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DOI：
10.3390/microorganisms8101535
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发表时间：
2020-10-06
期刊：
影响因子：
4.5
通讯作者：
Marzi A
中科院分区：
文献类型：
--
作者：
Furuyama W;Marzi A
Ebola virus (EBOV) is a highly pathogenic virus with human case fatality rates of up to 90%. EBOV uses transcriptional editing to express three different glycoproteins (GPs) from its GP gene: soluble GP (sGP), GP, and small sGP (ssGP). The molecular ratio of unedited to edited mRNA is about 70% (sGP): 25% (GP): 5% (ssGP), indicating that sGP is produced more abundantly than GP. While the presence of sGP has been confirmed in the blood during human EBOV infection, there is no report about its expression dynamics. In this study, we developed an EBOV-sGP-specific sandwich enzyme-linked immunosorbent assay (ELISA) using two different available antibodies and tested several animal serum samples to determine the concentration of sGP. EBOV-sGP was detected in nonhuman primate serum samples as early as 4 days after EBOV infection, correlating with RT-qPCR positivity. This ELISA might be further developed into a diagnostic tool for detection of EBOV in patients. Furthermore, this study provides insights into the expression dynamics of sGP during infection, which are important to decipher the function that sGP plays during infection.
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影响因子：
6.5
作者：
Coarsey CT;Esiobu N;Narayanan R;Pavlovic M;Shafiee H;Asghar W
通讯作者：
Asghar W
影响因子：
5.4
作者：
Hoenen, Thomas;Shabman, Reed S.;Feldmann, Heinz
通讯作者：
Feldmann, Heinz
影响因子：
8.8
作者：
Marzi, Andrea;Chadinah, Spencer;Feldmann, Heinz
通讯作者：
Feldmann, Heinz
影响因子：
28.3
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Goldstein T;Anthony SJ;Gbakima A;Bird BH;Bangura J;Tremeau-Bravard A;Belaganahalli MN;Wells HL;Dhanota JK;Liang E;Grodus M;Jangra RK;DeJesus VA;Lasso G;Smith BR;Jambai A;Kamara BO;Kamara S;Bangura W;Monagin C;Shapira S;Johnson CK;Saylors K;Rubin EM;Chandran K;Lipkin WI;Mazet JAK
通讯作者：
Mazet JAK
影响因子：
9.2
作者：
Furuyama, Wakako;Reynolds, Pierce;Marzi, Andrea
通讯作者：
Marzi, Andrea