Proteome profiling of heat, oxidative, and salt stress responses in Thermococcus kodakarensis KOD1.
Proteome profiling of heat, oxidative, and salt stress responses in Thermococcus kodakarensis KOD1.
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Thermococcus kodakarensis KOD1 热、氧化和盐应激反应的蛋白质组分析
DOI：
10.3389/fmicb.2015.00605
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发表时间：
2015
影响因子：
5.2
通讯作者：
Cheong GW
中科院分区：
文献类型：
--
作者：
Jia B;Liu J;Van Duyet L;Sun Y;Xuan YH;Cheong GW
嗜热种Thermococcus kodakarensis KOD 1是研究超嗜热菌的模式微生物，它适应了高温和高盐条件下的最佳生长。然而，该菌株的环境条件并不总是稳定的，该菌株可能面临不同的压力。在本研究中，我们比较了T。利用双向电泳技术对热、氧化和盐胁迫下的Kodakarensis进行了鉴定，并通过MALDI-TOF/MS对蛋白质点进行了鉴定。在上调的蛋白质中，有四种蛋白质（一种假设的蛋白质，吡哆醛生物合成裂解酶，过氧化物氧还蛋白和蛋白质二硫化物氧化还原酶）与所有三种压力。基因本体分析表明，这些蛋白质主要参与代谢和细胞过程。KEGG途径分析表明，这些酶的主要代谢途径与碳水化合物代谢、次生代谢产物合成和氨基酸生物合成有关。这些数据可能会增强我们对嗜热菌在极端环境中生存和适应的功能和分子机制的理解。
The thermophilic species, Thermococcus kodakarensis KOD1, a model microorganism for studying hyperthermophiles, has adapted to optimal growth under conditions of high temperature and salinity. However, the environmental conditions for the strain are not always stable, and this strain might face different stresses. In the present study, we compared the proteome response of T. kodakarensis to heat, oxidative, and salt stresses using two-dimensional electrophoresis, and protein spots were identified through MALDI-TOF/MS. Fifty-nine, forty-two, and twenty-nine spots were induced under heat, oxidative, and salt stresses, respectively. Among the up-regulated proteins, four proteins (a hypothetical protein, pyridoxal biosynthesis lyase, peroxiredoxin, and protein disulphide oxidoreductase) were associated with all three stresses. Gene ontology analysis showed that these proteins were primarily involved metabolic and cellular processes. The KEGG pathway analysis suggested that the main metabolic pathways involving these enzymes were related to carbohydrate metabolism, secondary metabolite synthesis, and amino acid biosynthesis. These data might enhance our understanding of the functions and molecular mechanisms of thermophilic Archaea for survival and adaptation in extreme environments.
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