Ganglion-specific splicing of TRPV1 underlies infrared sensation in vampire bats.
Ganglion-specific splicing of TRPV1 underlies infrared sensation in vampire bats.
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DOI：
10.1038/nature10245
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发表时间：
2011-08-03
期刊：
影响因子：
64.8
通讯作者：
Julius, David
中科院分区：
文献类型：
--
作者：
Gracheva, Elena O.;Cordero-Morales, Julio F.;Gonzalez-Carcacia, Jose A.;Ingolia, Nicholas T.;Manno, Carlo;Aranguren, Carla I.;Weissman, Jonathan S.;Julius, David
吸血蝙蝠（Desmodus rotundus）是专性吸血者，已经进化出专门的系统，以适应其独特的血腥生活方式。在这些适应中，最主要的是能够探测到红外辐射，以此作为定位温血猎物上热点的手段。在脊椎动物中，只有吸血蝙蝠、蟒蛇、蟒蛇和蝮蛇能够探测到红外辐射。在每一种情况下，红外线热量都是由三叉神经纤维检测到的，三叉神经纤维支配着动物脸上的专门的窝器官。因此，吸血蝙蝠和蛇通过开发专门的红外辐射探测系统，将温度感觉发挥到了极致。因此，这些生物为了解以物种或细胞类型特定方式进化调节温度感受器的分子和遗传机制提供了一个窗口。以前，我们已经表明，蛇增选一个非热敏通道（脊椎动物TRPA 1）产生红外探测器。在这里，我们发现吸血蝙蝠通过将其热激活阈值降低到~30°C来调节已经对热敏感的通道（TRPV 1）。这是通过选择性剪接TRPV 1转录本以产生具有截短的C末端胞质结构域的通道来实现的。值得注意的是，这些剪接事件只发生在三叉神经节（TG），而不是背根神经节（DRG），从而保持TRPV 1作为检测有害热在躯体传入的作用。这反映了一个独特的组织的蝙蝠TRPV 1基因，我们表明是特征的Laurasiatheria哺乳动物（牛，狗，鼹鼠），支持与蝙蝠密切的系统发育关系。这些发现揭示了一个独特的分子机制的生理调谐的热敏神经纤维。
Vampire bats (Desmodus rotundus) are obligate blood feeders that have evolved specialized systems to suit their unique sanguinary lifestyle . Chief among such adaptations is the ability to detect infrared radiation as a means of locating hot spots on warm-blooded prey. Among vertebrates, only vampire bats, boas, pythons, and pit vipers are capable of detecting infrared radiation . In each case, infrared heat is detected by trigeminal nerve fibers that innervate specialized pit organs on the animal’s face . Thus, vampire bats and snakes have taken thermosensation to the extreme by developing specialized systems for detecting infrared radiation. As such, these creatures provide a window into the molecular and genetic mechanisms underlying evolutionary tuning of thermoreceptors in a species or cell type specific manner. Previously, we have shown that snakes co-opt a non-heat sensitive channel (vertebrate TRPA1) to produce an infrared detector . Here we show that vampire bats tune an already heat sensitive channel (TRPV1) by lowering its thermal activation threshold to ~30°C. This is achieved through alternative splicing of TRPV1 transcripts to produce a channel with a truncated C-terminal cytoplasmic domain. Remarkably, these splicing events occur exclusively in trigeminal ganglia (TG), and not dorsal root ganglia (DRG), thereby maintaining a role for TRPV1 as a detector of noxious heat in somatic afferents. This reflects a unique organization of the bat TRPV1 gene that we show to be characteristic of Laurasiatheria mammals (cows, dogs, and moles), supporting a close phylogenetic relationship with bats. These findings reveal a unique molecular mechanism for physiological tuning of thermosensory nerve fibers.
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影响因子：
56.9
作者：
Murphy, WJ;Eizirik, E;Springer, MS
通讯作者：
Springer, MS
DOI：
10.1073/pnas.0603797103
发表时间：
2006-06-27
影响因子：
11.1
作者：
Nishihara, Hidenori;Hasegawa, Masarni;Okada, Norihiro
通讯作者：
Okada, Norihiro
影响因子：
2.5
作者：
MOLENAAR, GJ
通讯作者：
MOLENAAR, GJ
影响因子：
4.5
作者：
SCHAFER, K;BRAUN, HA;KURTEN, L
通讯作者：
KURTEN, L
影响因子：
--
作者：
Tellgren-Roth, Asa;Dittmar, Katharina;Liberles, David A.
通讯作者：
Liberles, David A.