Comparative transcriptome analysis of stylar canal cells identifies novel candidate genes implicated in the self-incompatibility response of Citrus clementina.
Comparative transcriptome analysis of stylar canal cells identifies novel candidate genes implicated in the self-incompatibility response of Citrus clementina.
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DOI：
10.1186/1471-2229-12-20
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发表时间：
2012-02-14
影响因子：
5.3
通讯作者：
Gentile A
中科院分区：
文献类型：
--
作者：
Caruso M;Merelo P;Distefano G;La Malfa S;Lo Piero AR;Tadeo FR;Talon M;Gentile A
柑橘的生殖生物学仍然知之甚少。虽然近年来已经作出了一些努力来研究花粉雌蕊相互作用和自交不亲和性，很少有信息是关于调控这些过程的分子机制。本文报道了柑橘花粉雌蕊互作和自交不亲和性的候选基因的鉴定。这些基因是通过比较激光显微切割的花柱管细胞（SCC）的转录组而鉴定出来的，这些细胞是从两种自交不亲和反应不同的基因型（“Comune”，自交不亲和品种和“Monreal”，“Comune”的自交不亲和突变）中分离出来的。SCC的转录组分析表明，少数特定的差异调节，主要是未知的转录本与“Monreal”自交不亲和性的崩溃。其中，一个新的F-box基因在激光显微切割的花柱管细胞和自花授粉的完整花柱中表现出强烈的上调，伴随着花粉管生长的停滞。此外，我们确定了一个非特征性的基因家族密切相关的自交不亲和遗传程序中激活的“Comune”。三种不同的富含天冬氨酸（Asp-rich）的蛋白质基因，位于克莱门汀基因组中的串联，在'Comune'的转录组中过度表达。这些基因与DELLA基因紧密相连，此前发现DELLA基因在花粉-雌蕊相互作用期间在自交不亲和基因型中上调。花柱管细胞的高度特异性的转录组调查确定了新的基因，这些基因以前在柑橘和其他植物物种中与自花花粉排斥无关。生物信息学和转录分析表明，导致“Monreal”自交亲和性的突变影响了位于限制性基因组区域的非同源基因的表达。此外，我们推测，富含天冬氨酸蛋白基因可能作为Ca 2+的“截留”蛋白，潜在地调节Ca 2+的自花粉识别过程中的稳态。
Reproductive biology in citrus is still poorly understood. Although in recent years several efforts have been made to study pollen-pistil interaction and self-incompatibility, little information is available about the molecular mechanisms regulating these processes. Here we report the identification of candidate genes involved in pollen-pistil interaction and self-incompatibility in clementine (Citrus clementina Hort. ex Tan.). These genes have been identified comparing the transcriptomes of laser-microdissected stylar canal cells (SCC) isolated from two genotypes differing for self-incompatibility response ('Comune', a self-incompatible cultivar and 'Monreal', a self- compatible mutation of 'Comune'). The transcriptome profiling of SCC indicated that the differential regulation of few specific, mostly uncharacterized transcripts is associated with the breakdown of self-incompatibility in 'Monreal'. Among them, a novel F-box gene showed a drastic up-regulation both in laser microdissected stylar canal cells and in self-pollinated whole styles with stigmas of 'Comune' in concomitance with the arrest of pollen tube growth. Moreover, we identify a non-characterized gene family as closely associated to the self-incompatibility genetic program activated in 'Comune'. Three different aspartic-acid rich (Asp-rich) protein genes, located in tandem in the clementine genome, were over-represented in the transcriptome of 'Comune'. These genes are tightly linked to a DELLA gene, previously found to be up-regulated in the self-incompatible genotype during pollen-pistil interaction. The highly specific transcriptome survey of the stylar canal cells identified novel genes which have not been previously associated with self-pollen rejection in citrus and in other plant species. Bioinformatic and transcriptional analyses suggested that the mutation leading to self-compatibility in 'Monreal' affected the expression of non-homologous genes located in a restricted genome region. Also, we hypothesize that the Asp-rich protein genes may act as Ca2+ "entrapping" proteins, potentially regulating Ca2+ homeostasis during self-pollen recognition.
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影响因子：
5.3
作者：
Agustí J;Merelo P;Cercós M;Tadeo FR;Talón M
通讯作者：
Talón M
影响因子：
4.3
作者：
Golisz, Anna;Sugano, Mami;Fujii, Yoshiharu
通讯作者：
Fujii, Yoshiharu
DOI：
10.1073/pnas.162339999
发表时间：
2002-08-20
影响因子：
11.1
作者：
Gagne, JM;Downes, BP;Vierstra, RD
通讯作者：
Vierstra, RD
影响因子：
3.5
作者：
Gentile, Alessandra;Antognoni, Fabiana;Del Duca, Stefano
通讯作者：
Del Duca, Stefano
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.