Hearing Without Neuroglobin
Hearing Without Neuroglobin
复制标题
没有神经球蛋白的听力
DOI：
10.1016/j.neuroscience.2017.10.010
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发表时间：
2017
期刊：
影响因子：
3.3
通讯作者：
Reuss S
中科院分区：
文献类型：
--
作者：
Nowotny M;Kiefer L;Andre D;Fabrizius A;Hankeln T;Reuss S
神经珠蛋白（Neuroglobin, Ngb）是呼吸系统蛋白珠蛋白家族的一员，近年来在听觉通路的许多神经元中都有发现。然而，到目前为止，对Ngb在听力过程中的作用还一无所知。因此，我们通过记录野生型（C57BL/6N）和ngb敲除小鼠的畸变产物耳声发射（DPOAE）和听觉脑干反应（ABRs）来研究听觉功能。在KO小鼠中，DPOAE阈值在5-18 kHz范围内适度增加，在8和10 kHz范围内达到统计学意义，而ABR阈值在组间无差异。通过给对侧耳额外的噪声激活传出系统，仅在WT动物中导致f2 - f1发射水平增加。噪声暴露导致两组动物DPOAE和ABR的急性阈值变化相似。以DPOAE阈值降低表示的听力功能恢复在4天后和4周后各组间无显著差异。ABR记录显示，噪音创伤引起的阈值变化在野生型小鼠中略有改善。虽然在噪声过度暴露前两组的ABR振幅相似，但在创伤后四周，在KO小鼠中观察到最新峰对峰反应幅度（起源于下丘）的中度但具有统计学意义的下降。我们的研究结果表明，至少在本研究中使用的模型中，缺乏Ngb只会导致听力能力的轻微缺陷。Ngb在传出系统中可能的功能作用值得进一步研究。
Neuroglobin (Ngb) is a member of the globin family of respiratory proteins, which was recently observed in many neurons of the auditory pathways. Up to now, however, nothing was known about the role of Ngb in hearing processes. We therefore studied auditory function by recording distortion-product otoacoustic emissions (DPOAE) and auditory brainstem responses (ABRs) in wild-type (C57BL/6N) and Ngb-knockout mice. In KO mice, DPOAE thresholds were moderately augmented in the range of 5–18 kHz, reaching statistical significance at 8 and 10 kHz, while the ABR thresholds were not different between groups. The activation of the efferent system by an additional noise given to the contralateral ear resulted in an increasedf2–f1-emission level only in WT animals. A noise exposure resulted in similar acute threshold shifts in the DPOAE and ABR of both animal groups. The recovery of hearing function, expressed by decreased DPOAE thresholds, was not significantly different between groups after four days and after four weeks. ABR recordings showed that threshold shifts elicited by noise-trauma were slightly better revised in wild-type mice. While ABR amplitudes were similar in both groups before noise overexposure, four weeks after trauma a moderate but statistically significant decrease of the latest peak-to-peak response amplitude (originating in the inferior colliculus) was observed in KO mice. Our results suggest that the lack of Ngb, at least in the model used in the present study, results in only marginal deficits in hearing ability. A putative functional role of Ngb in the efferent system warrants further studies.
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发表时间：
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期刊：
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