Reversion of Gut Microbiota during the Recovery Phase in Patients with Asymptomatic or Mild COVID-19: Longitudinal Study.
Reversion of Gut Microbiota during the Recovery Phase in Patients with Asymptomatic or Mild COVID-19: Longitudinal Study.
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DOI：
10.3390/microorganisms9061237
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发表时间：
2021-06-07
期刊：
影响因子：
4.5
通讯作者：
Park SK
中科院分区：
文献类型：
--
作者：
Kim HN;Joo EJ;Lee CW;Ahn KS;Kim HL;Park DI;Park SK
据报道，新冠肺炎患者会出现胃肠道症状和呼吸道症状，但新冠肺炎对肠道微生物区系的影响尚不清楚。我们研究了无症状或轻度新冠肺炎患者在恢复期与SARS-CoV-2呼吸道感染相关的肠道微生物群谱。对相同的患者进行了纵向分析，以确定从新冠肺炎康复后肠道微生物区系是否发生了变化。我们应用16SRRNA扩增序列分析了12例无症状或轻度新冠肺炎患者的两份粪便样本。在两个时间点采集粪便样本：SARS-CoV-2感染期间(感染状态)和病毒RNA阴性转换后(恢复状态)。我们还将微生物组数据与36名健康对照组的数据进行了比较。与感染状态相比，恢复状态的微生物均匀度显著增加。SARS-CoV-2感染导致类杆菌的枯竭，而在恢复状态下观察到大量的类杆菌并有迅速逆转的趋势。感染状态下的细菌/类杆菌比值明显高于恢复状态。即使是无症状或轻度新冠肺炎的患者感染后也可观察到肠道微生物群的失调，而SARS-CoV-2RNA阴性后肠道微生物区系的组成已恢复。修饰肠道微生物以回应新冠肺炎可能是一种有用的治疗替代方案。
Patients with COVID-19 have been reported to experience gastrointestinal symptoms as well as respiratory symptoms, but the effects of COVID-19 on the gut microbiota are poorly understood. We explored gut microbiome profiles associated with the respiratory infection of SARS-CoV-2 during the recovery phase in patients with asymptomatic or mild COVID-19. A longitudinal analysis was performed using the same patients to determine whether the gut microbiota changed after recovery from COVID-19. We applied 16S rRNA amplicon sequencing to analyze two paired fecal samples from 12 patients with asymptomatic or mild COVID-19. Fecal samples were selected at two time points: during SARS-CoV-2 infection (infected state) and after negative conversion of the viral RNA (recovered state). We also compared the microbiome data with those from 36 healthy controls. Microbial evenness of the recovered state was significantly increased compared with the infected state. SARS-CoV-2 infection induced the depletion of Bacteroidetes, while an abundance was observed with a tendency to rapidly reverse in the recovered state. The Firmicutes/Bacteroidetes ratio in the infected state was markedly higher than that in the recovered state. Gut dysbiosis was observed after infection even in patients with asymptomatic or mild COVID-19, while the composition of the gut microbiota was recovered after negative conversion of SARS-CoV-2 RNA. Modifying intestinal microbes in response to COVID-19 might be a useful therapeutic alternative.
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DOI：
10.1126/science.aam9949
发表时间：
2017-08-11
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Byndloss MX;Olsan EE;Rivera-Chávez F;Tiffany CR;Cevallos SA;Lokken KL;Torres TP;Byndloss AJ;Faber F;Gao Y;Litvak Y;Lopez CA;Xu G;Napoli E;Giulivi C;Tsolis RM;Revzin A;Lebrilla CB;Bäumler AJ
通讯作者：
Bäumler AJ
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
168.9
作者：
Huang, Chaolin;Wang, Yeming;Cao, Bin
通讯作者：
Cao, Bin
影响因子：
8.8
作者：
Sencio, Valentin;Barthelemy, Adeline;Trottein, Francois
通讯作者：
Trottein, Francois
DOI：
10.3138/jammi-2020-0030
发表时间：
2020-12-01
期刊：
Journal of the Association of Medical Microbiology and Infectious Disease Canada = Journal officiel de l'Association pour la microbiologie medicale et l'infectiologie Canada
影响因子：
--
作者：
Byambasuren, Oyungerel;Cardona, Magnolia;Glasziou, Paul
通讯作者：
Glasziou, Paul